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Title Stem cell tools and other experimental protocols / edited by Irina Klimanskaya, Robert Lanza
Published Amsterdam ; Boston : Elsevier Academic Press, [2006]
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Description 1 online resource (xxxviii, 477 pages, 22 unnumbered pages of plates) : illustrations (some color)
Series Methods in enzymology, 0076-6879 ; v. 420
Methods in enzymology ; v. 420. 0076-6879
Contents In vitro experimentation and research tools: Human embryo culture / A. Mercader ... [et al.] -- Characterization and evaluation of human embryonic stem cells / C. Xu ... [et al.] -- Feeder-free culture of human embryonic stem cells / M. Amit ... [et al.] -- Transgene expression and RNA interference in embryonic stem cells / H. Zaehres ... [et al.] -- Lentiviral vector-mediated gene delivery into human embryonic stem cells / M. Gropp ... [et al.] -- The use of retroviral vectors for gene transfer into hematopoietic stem cells / J. Holst ... [et al.] -- Engineering embryonic stem cells with recombinase systems / F. Schnutgen ... [et al.] -- Gene trapping in embryonic stem cells / W.L. Stanford ... [et al.] -- Genechips in stem cell research / J. Hipp ... [et al.] -- Microarray analysis of stem cells and differentiation / H.Y. Chang ... [et al.] -- Purification of hematopoietic stem cells using the side population / K.K. Lin ... [et al.] -- Cellular reprogramming / S. Agarwal ... [et al.] -- Tissue engineering and regenerative medicine: Tissue engineering using adult stem cells / D. Eberli ... [et al.] -- Tissue engineering using human embryonic stem cells / S. Cohen ... [et al.] -- Engineering cardiac tissue from embryonic stem cells / X. Guo ... [et al.] -- Mesenchymal stem cells and tissue engineering / N.W. Marion ... [et al.] -- Bone reconstruction with bone marrow stromal cells / W. Liu ... [et al.] -- Engineering three-dimensional tissue structures using stem cells / J. Zoldan ... [et al.] -- Immunogenicity of embryonic stem cells and their progeny / M. Drukker ... [et al.] -- Manufacturing considerations for clinical uses of therapies derived from stem cells / D.J. Weber
Summary This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook" and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conven
Bibliography Includes bibliographical references and indexes
Notes Description based on print version record
Subject Stem cells -- Research -- Handbooks, manuals, etc.
Stem cells.
Tissue engineering.
Stem Cells.
Tissue Engineering.
Genre/Form Handbooks and manuals.
Form Electronic book
Author Klimanskaya, Irina.
Lanza, R. P. (Robert Paul), 1956-
ISBN 0080469647 (electronic bk.)
012373651X (electronic bk.)
9780080469645 (electronic bk.)
9780123736512 (electronic bk.)