| Description |
1 online resource (272 pages) |
| Contents |
Cover; Title Page; Copyright Page; Preface; The Author; Cell Physiology and Genetics of Higher Plants; Contents; Chapter 1 Protoplast â#x80;#x94; Isolation and Cell Regeneration; I. Definition and Significance; II. Isolation; A. Methods of Isolation; 1. Mechanical Isolation; 2. Enzymatic Isolation; a. Steps in Enzymatic Isolation; B. Factors Affecting Isolation; 1. Source Material; a. Mesophyll Tissue; b. Callus and Cell Suspension; c. Pollen Grains; 2. Osmoticum; 3. Enzymes; 4. Isolation Medium; 5. Incubation Conditions; 6. Tissue Pretreatment; III. Harvest, Purification, and Viability |
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B. Protoplast Culture of Monocot PlantsIX. Protoplast to Plant; X. Microplasts/Cytoplasts and their Utility; XI. Protoplast â#x80;#x94; Applications in Fundamental Research; A. Isolation of Cell Components; B. Photosynthesis; C. Stomatal Physiology; D. Environmental Monitoring; E. Cell Wall Formation; F. Herbicide Physiology; G. Circadian Rhythm; H. Nitrogen Metabolism; I. Hormone Physiology; References; 2. Cell Modification; I. Introduction; II. Cell Genetics â#x80;#x94; Complementary to Conventional Genetics; III. Terminology â#x80;#x94; Mutant vs. Variant; A. Epigenetic Variation; B. Mutation |
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C. Limits to SelectionVII. System; A. Necessity of Plating Technique; B. Mutagen Treatment; C. Mutation Frequency; D. Ploidy of Plant Cells; E. Model System; VIII. Variant Cell Lines; A. Pigment Variants; B. Alkaloid Variants; C. Antibiotic Resistance; D. Resistance to Purine and Pyrimidine Analogues; E. Resistance to Amino Acid Analogues; F. Resistance to Toxins; G. Resistance to Herbicides; H. Resistance to Stress; 1. Chilling Stress; 2. Heat Stress; 3. Salt Stress; 4. Drought Stress; 5. Metal Stress; I. Developmental Variants; 1. Growth Regulator Variants; 2. Transcriptional Variants |
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IV. Protoplast to Cell â#x80;#x94; A TransitionV. Culture; A. Methods of Culture; 1. Suspension Culture; a. Single Drop or Multiple Drops; b. Microchamber Culture; c. Microdroplet Culture; 2. Plating of Protoplasts; VI. Cell Regeneration; A. Cell Wall Formation; B. Cell Division; C. Factors Affecting Division; 1. Physical Environment; a. Density; b. Light; c. Temperature; 2. Chemical Environment; a. Nutrient Medium; b. Osmoticum and Carbon Source; c. Hormones and Growth Regulators; d. pH; 3. Genetic Nature; VII. Culture at Low Density; VIII. Problems in Protoplast Culture; A. General |
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IV. Spontaneous Modification or Somaclonal VariationA. Variant Plants with Desirable Characters; 1. Sugarcane; 2. Potato; 3. Maize; 4. Alfalfa; 5. Geranium; 6. Brassica; B. Variant Plants with New Characters; 1. Tobacco; 2. Rice; 3. Wheat; 4. Tomato; C. Variants with Chromosome Modifications; D. Nature of Variation; 1. Is Variation Preexisting?; 2. Is Variation Simple or Complex?; 3. Is Variation Heritable?; E. Origin of Variation; F. Limitations and Prospects; V. Induced Modification; VI. Selection; A. Direct Selection for Resistance; B. Indirect Selection for Conditional Mutants |
| Notes |
J. Metabolic Variants |
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Print version record |
| Subject |
Plant cells and tissues.
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Plant cytogenetics.
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Plant cells and tissues.
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Plant cytogenetics.
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| Form |
Electronic book
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| ISBN |
1351087371 |
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9781351087377 |
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