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Title Nuclear transfer protocols : cell reprogramming and transgenesis / edited by Paul J. Verma, Alan O. Trounson
Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xi, 337 pages) : illustrations
Series Methods in molecular biology, 1064-3745 ; 348
Methods in molecular biology (Clifton, N.J.) ; v. 348
Contents Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in non-human primates / Shoukhrat M. Mitalipov and Don P. Wolf
Cloning of exotic/endangered species : desert bighorn sheep / James "Buck" Williams [and others] -- Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris [and others] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien [and others] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki [and others] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson
Summary Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer. Of immense benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Cell nuclei -- Transplantation.
Gene therapy.
Transgenic animals.
Nuclear Transfer Techniques
Cloning, Organism -- methods
Cell Differentiation -- genetics
Cell Nucleus -- physiology
Gene Transfer Techniques
Genetic Therapy
Animals, Genetically Modified
MEDICAL -- Research.
Cell nuclei -- Transplantation
Gene therapy
Transgenic animals
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Verma, Paul J.
Trounson, Alan
ISBN 1588292800
1597451541
9781597451543
9781588292803
1280832444
9781280832444
9786610832446
6610832447