Title Receptor binding techniques / edited by Anthony P. Davenport

Edition 2nd ed Published Totowa, N.J. : Humana Press, ©2005

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Description 1 online resource (x, 240 pages) : illustrations Series Methods in molecular biology ; v. 306 Methods in molecular biology (Clifton, N.J.) ; v. 306 Contents Receptor databases and computational websites for ligand binding -- Identification of orphan g protein-coupled receptor ligands using fluorometric imaging plate reader assays -- Quantitative analysis of orphan g protein-coupled receptor -- Mrnas by taqman real-time pcr: g2a and gpr4 lysophospholipid receptor expression in -- Leucocytes and in a rat myocardial infarction-heart model -- -- Mrna detection by in situ and northern hybridization -- Radioligand binding and molecular imaging techniques for the quantitative analysis of established and emerging orphan receptor systems -- Measurement of radioligand binding by scintillation proximity assay -- Autoradiography of enzymes, second messenger systems, and ion channels -- Immunocytochemical localization of receptors using light and confocal microscopy with applications to the phenotypic characterization of knockout mice -- live cell imaging of g protein-coupled receptors -- imaging and characterization of radioligands for positron emission tomography using quantitative phosphor imaging autoradiography -- dynamic in vivo imaging of receptors in small animals using positron emission tomography Summary Maintaining the high standards set by the successful first edition, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical industry and academia take advantage of the latest developments to provide detailed practical methods for studying receptors in silico, in vitro, and in vivo. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knock-out mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan polymerase chain reaction (PCR). These ligand binding techniques are perfect for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Receptor Binding Techniques, Second Edition offers academic and commercial researchers in the pharmaceutical and biotechnology industries a set of proven techniques for the successful characterization of receptors and the phenotyping of transgenic animals, including knock-outs Analysis receptoren receptors biochemische receptoren biochemical receptors liganden ligands messenger rna eiwitten proteins biologische technieken biological techniques protocollen protocols Molecular Biology (General) Moleculaire biologie (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Receptor-ligand complexes -- Research -- Laboratory manuals Radioligand assay -- Laboratory manuals Cell receptors -- Research -- Laboratory manuals Binding sites (Biochemistry) Binding Sites Radioligand Assay -- methods Receptors, Drug -- isolation & purification SCIENCE -- Life Sciences -- Cell Biology. Binding sites (Biochemistry) Cell receptors -- Research Radioligand assay Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Davenport, Anthony P. LC no. 2004025577 ISBN 1592599273 9781592599271 158829420X 9781588294203

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