| Description |
1 online resource (xiv, 410 pages) : illustrations |
| Contents |
CHAPTER 1 Introduction; 1 The Electron Microscope and Biology; 1.1 General Remarks; 1.2 Three-Dimensional Electron Microscopy; 2 Single-Particle Versus Crystallographic Analysis; 3 Crystallography without Crystals; 4 Toward a Unified Approach to Structural Analysis of Macromolecules; 5 Single-Particle Reconstruction, Macromolecular Machines, and Structural Proteomics; 6 The Electron Microscope and the Computer; CHAPTER 2 Electron Microscopy of Macromolecular Assemblies; 1 Principle of the Transmission Electron Microscope; 2 Specimen Preparation Methods; 2.1 Introduction |
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2.2 Negative Staining2.3 Glucose Embedment; 2.4 Use of Tannic Acid; 2.5 Ice-Embedded Specimens; 2.6 Hybrid Techniques: Cryo-Negative Staining; 2.7 Labeling with Gold Clusters; 2.8 Support Grids; 3 Principle of Image Formation in the Transmission Electron Microscope; 3.1 Introduction; 3.2 The Weak-Phase Object Approximation; 3.3 The Contrast Transfer Theory; 3.4 Amplitude Contrast; 3.5 Formulation of Bright-Field Image Formation Using Complex Atomic Scattering Amplitudes; 3.6 Optical and Computational Diffraction Analysis-The Power Spectrum; 3.7 Determination of the Contrast Transfer Function |
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3.8 Instrumental Correction of the Contrast Transfer Function3.9 Computational Correction of the Contrast Transfer Function; 3.10 Locally Varying CTF and Image Quality; 4 Special Imaging Techniques and Devices; 4.1 Low-Dose Electron Microscopy; 4.2 Spot Scanning; 4.3 Energy Filtration; 4.4 Direct Image Readout and Automated Data Collection; CHAPTER 3 Two-Dimensional Averaging Techniques; 1 Introduction; 1.1 The Different Sources and Types of Noise; 1.2 Principle of Averaging: Historical Notes; 1.3 Equivalence between Averaging and Quasi-Optical Fourier Filtration |
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1.4 A Discourse on Terminology: Views Versus Projections1.5 The Role of Two-Dimensional Averaging in the Three-Dimensional Analysis of Single Molecules; 1.6 Origins of Orientational Preferences; 2 Digitization and Selection of Particles; 2.1 Hardware for Digitization; 2.2 The Sampling Theorem; 2.3 Interactive Particle Selection; 2.4 Automated Particle Selection; 3 Alignment Methods; 3.1 Quantitative Definitions of Alignment; 3.2 Homogeneous Versus Heterogeneous Image Sets; 3.3 Translational and Rotational Cross-Correlation; 3.4 Reference-Based Alignment Techniques |
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3.5 Reference-Free Alignment Techniques3.6 Alignment Using the Radon Transform; 4 Averaging and Global Variance Analysis; 4.1 The Statistics of Averaging; 4.2 The Variance Map and the Analysis of Statistical Significance; 4.3 Signal-to-Noise Ratio; 5 Resolution; 5.1 The Concept of Resolution; 5.2 Resolution Criteria; 5.3 Resolution and Cross-Resolution; 5.4 Resolution-Limiting Factors; 5.5 Statistical Requirements following the Physics of Scattering; 5.6 Noise Filtering; 6 Validation of the Average Image; CHAPTER 4 Multivariate Data Analysis and Classification of Images; 1 Introduction |
| Summary |
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics & structural biology at present |
| Bibliography |
Includes bibliographical references (pages 345-398) and index |
| Notes |
English |
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Print version record |
| Subject |
Three-dimensional imaging in biology.
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Electron microscopy.
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Microscopy, Electron
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Microscopy, Electron -- methods
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Imaging, Three-Dimensional
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Imaging, Three-Dimensional -- methods
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Macromolecular Substances -- analysis
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Image Processing, Computer-Assisted
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electron microscopy.
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NATURE -- Reference.
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SCIENCE -- Life Sciences -- General.
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SCIENCE -- Life Sciences -- Biology.
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Electron microscopy
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Three-dimensional imaging in biology
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Elektronenmicroscopie.
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Driedimensionale afbeeldingen.
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Macromoleculen.
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| Form |
Electronic book
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| LC no. |
2004018762 |
| ISBN |
9780198034384 |
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0198034385 |
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1429415215 |
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9781429415217 |
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9780195150964 |
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0195150961 |
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9780195182187 |
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0195182189 |
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1280845511 |
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9781280845512 |
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