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Book Cover
Author Amberg, David C.

Title Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual / David C. Amberg, Daniel J. Burke, Jeffrey N. Strathern
Edition 2005 edition
Published Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, [2005]


Location Call no. Vol. Availability
 W'PONDS  579.562135 Bur/Miy 2005  AVAILABLE
Description xvii, 230 pages : illustrations
Contents Looking at yeast cells -- Isolation and characterization of auxotrophic, temperature-sensitive, and osmotic-sensitive mutants -- Meiotic mapping -- Mitotic recombination and random spore analysis -- Transformation of yeast -- Synthetic lethal mutants -- Gene replacement -- Isolation of ras2 suppressors -- Manipulating cell types -- Isolating mutants by insertional mutagenesis -- Isolation of separation of function mutants by two-hybrid differential interaction screening -- High-efficiency transformation of yeast -- "Quick and dirty" plasmid transformation of yeast colonies -- Yeast DNA isolations -- Yeast protein extracts -- TAP purification -- Yeast RNA isolations -- Hydroxylamine mutagenesis of plasmid DNA -- Assay of beta-galactosidase in yeast -- Plate assay for carboxypeptidase Y -- Random spore analysis -- Yeast vital stains -- Yeast immunofluorescence -- Actin staining in fixed cells -- PCR-mediated gene disruption -- Yeast colony PCR -- Measuring yeast cell density by spectrophotometry -- Cell synchrony -- Chromatin immunoprecipitation -- Flow cytometry of yeast DNA -- Logarithmic growth -- EMS mutagenesis -- Tetrad dissection -- Making a tetrad dissection needle -- Picking zygotes -- Determining plating efficiency -- DNA miniprep from e. coli -- Preparing and transforming competent e. coli -- Storing and handling the systematic deletion collection -- Media -- Stock preservation -- Yeast genetic and physical maps -- Templates for making streak plates -- Electrophoretic karyotypes of strains for southern blot mapping -- Strains -- Counting yeast cells with a standard hemocytometerchamber -- Tetrad scoring sheet
Notes Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000
Bibliography Includes bibliographical references
Author Strathern, Jeffrey N.
Burke, Dan, 1954-
Amberg, David C.
Burke, Dan, 1954- Methods in yeast genetics
Cold Spring Harbor Laboratory.
LC no. 2004028765
ISBN 0879697288 paperback
Other Titles Yeast genetics