| Description |
1 online resource (xiv, 486 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1657 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1657. 1064-3745
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| Contents |
Discovery of the second messenger Cyclic di-GMP / Ute Romling and Michael Y. Galperin -- Enzymatic production of c-di-GMP using a thermophilic diguanylate cyclase / Prabhadevi Venkataramani and Zhao-Xun Liang -- Synthesis of [32P]-c-di-GMP for diguanylate cyclase and phosphodiesterase activity determinations / Barbara I. Kazmierczak -- High-performance liquid chromatography (HPLC)-based detection and quantitation of cellular c-di-GMP / Olga E. Petrova and Karin Sauer -- Identification and quantification of cyclic di-guanosine monophosphate and its linear metabolites by reversed-phase LC-MS/MS / Heike Bahre and Volkhard Kaever -- Detection of cyclic dinucleotides by STING / Xiao-Xia Du and Xiao-Dong Su -- Spectrophotometric and mass spectroscopic methods for the quantification and kinetic evaluation of in vitro c-di-GMP synthesis / Geoffrey B. Severin and Christopher M. Waters -- Gauging and visualizing c-di-GMP levels in Pseudomonas aeruginosa using fluorescence-based biosensors / Morten Rybtke, Song Lin Chua, Joey Kuok Hoong Yam, Michael Givskov, Liang Yang, and Tim Tolker-Nielsen -- Cyclic di-GMP-responsive transcriptional reporter bioassays in Pseudomonas aeruginosa / Bradley R. Borlee, Grace I. Borlee, Kevin H. Martin, and Yasuhiko Irie -- Live flow cytometry analysis of c-di-GMP levels in single cell populations / ongchan Yeo, Xin C. Wang, and Ming C. Hammond -- Experimental detection and visualization of the extracellular matrix in macrocolony biofilms / Diego O. Serra and Regine Hengge -- Congo red stain identifies matrix overproduction and is an indirect measurement for c-di-GMP in many species of bacteria / Christopher J. Jones and Daniel J. Wozniak -- Type IV Pili-dependent motility as a tool to determine the activity of c-di-GMP modulating enzymes in Myxococcus xanthus / Dorota Skotnicka and Lotte Søgaard-Andersen -- Using light-activated enzymes for modulating intracellular c-di-GMP levels in bacteria / Min-Hyung Ryu, Anastasia Fomicheva, Lindsey O'Neal, Gladys Alexandre, and Mark Gomelsky -- Analysis of c-di-GMP levels synthesized by a photoreceptor protein in response to different light qualities using an in vitro enzymatic assay / Veronika Angerer, Lars-Oliver Essen, and Annegret Wilde -- Probing the role of cyclic di-GMP signaling systems in disease using Chinese radish / Shi-Qi An, Ji-Liang Tang, and J. Maxwell Dow -- Contribution of cyclic di-GMP in the control of type III and type VI secretion in Pseudomonas aeruginosa / Ronan R. McCarthy, Martina Valentini, and Alain Filloux -- Semiquantitative analysis of the red, dry, and rough colony morphology of Salmonella enterica serovar typhimurium and Escherichia coli using congo red / Annika Cimdins and Roger Simm -- Fluorescent 2-aminopurine c-di-GMP and GpG analogs as PDE probes / Jie Zhou, Clement Opoku-Temeng, and Herman O. Sintim -- Measuring cyclic diguanylate (c-di-GMP)-specific phosphodiesterase activity Using the MANT-c-di-GMP assay / Dorit Eli, Trevor E. Randall, Henrik Almblad, Joe J. Harrison, and Ehud Banin -- Determining phosphodiesterase activity (radioactive assay) / Barbara I. Kazmierczak -- Determining diguanylate cyclase activity (radioactive assay) / Barbara I. Kazmierczak -- Detection of c-di-GMP-responsive DNA binding / Jacob R. Chambers and Karin Sauer -- Use of nonradiochemical DNAse footprinting to analyze c-di-GMP modulation of DNA-binding proteins / Claudine Baraquet and Caroline S. Harwood -- Detection of cyclic di-GMP binding proteins utilizing a biotinylated cyclic di-GMP pull-down assay / Jacob R. Chambers and Karin Sauer -- Probing protein-protein interactions with genetically encoded photoactivatable cross-linkers / Richard B. Cooley and Holger Sondermann -- Identification of c-di-AMP-binding proteins using magnetic beads / Jan Kampf, Jan Gundlach, Christina Herzberg, Katrin Treffon, and Jorg Stulke -- Pull-down with a c-di-GMP-specific capture compound coupled to mass spectrometry as a powerful tool to identify novel effector proteins / Benoıt-Joseph Laventie, Timo Glatter, and Urs Jenal -- Identification of c-di-GMP-responsive riboswitches / Johann Peltier and Olga Soutourina -- Isothermal titration calorimetry to determine apparent dissociation constants (Kd) and stoichiometry of interaction (n) of C-di-GMP binding proteins / Bruno Y. Matsuyama, Petya V. Krasteva, and Marcos V.A.S. Navarro -- Targeting c-di-GMP signaling, biofilm formation, and bacterial motility with small molecules / Clement Opoku-Temeng and Herman O. Sintim -- Discovering selective diguanylate cyclase inhibitors : from PleD to discrimination of the active site of Cyclic-di-GMP phosphodiesterases / S. Rinaldo, G. Giardina, F. Mantoni, A. Paiardini, Alessio Paone, and Francesca Cutruzzola -- High-throughput screening for compounds that modulate the cellular c-di-GMP level in bacteria / Julie Groizeleau, Jens Bo Andersen, Michael Givskov, Jens Berthelsen, and Tim Tolker-Nielsen -- Genetic tools to study c-di-GMP-dependent signaling in Pseudomonas aeruginosa / Livia Leoni, Sarika Vishnu Pawar, and Giordano Rampioni |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocols, viewed September 11, 2017) |
| Subject |
Cyclic guanylic acid -- Laboratory manuals
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Cyclic guanylic acid.
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Cyclic GMP
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Cyclic guanylic acid
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Sauer, Karin, editor
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| ISBN |
9781493972401 |
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1493972405 |
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