| Description |
1 online resource (streaming video file) (750 secs.) |
| Series |
Biology |
| Summary |
We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER+ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ Kd measurement and calibration is also discussed |
| Audience |
For undergraduate, graduate, and professional students |
| Notes |
English |
|
Description based on online resource; title from resource description page |
| Subject |
Biology
|
| Form |
Streaming audio
|
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