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Title Plant cell culture protocols / edited by Victor M. Loyola-Vargas, Neftalí Ochoa-Alejo
Edition Fourth edition
Published New York : Humana Press : Springer, [2018]
©2018

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Description 1 online resource (xv, 507 pages) : illustrations (chiefly color)
Series Methods in molecular biology, 1064-3745 ; v. 1815
Methods in molecular biology (Clifton, N.J.) ; v. 1815.
Contents Introduction to plant tissue culture : advances and perspectives / Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo -- Micropropagation in the twenty-first century / Jean Carlos Cardoso, Lee Tseng Sheng Gerald, and Jaime A. Teixeira da Silva -- Cellular and morpho-histological foundations of in vitro plant regeneration / Diego Ismael Rocha, Lorena Melo Vieira, Andréa Dias Koehler, and Wagner Campos Otoni -- Bacterial endophytes in plant tissue culture : mode of action, detection, and control / Mona Quambusch and Traud Winkelmann -- Digital photography as a tool of research and documentation in plant tissue culture / Victor Gaba, Yehudit Tam, Danny Shavit, and Benjamin Steinitz -- Selection of molecular markers for the estimation of somaclonal variation / Octavio Martínez -- Plant tissue culture : a battle horse in the genome editing using CRISPR/Cas9 / Víctor M. Loyola-Vargas and Randy N. Avilez-Montalvo -- Micropropagation of agave species / Benjamín Rodríguez-Garay and José Manuel Rodríguez-Domínguez -- Protocol for the micropropagation of coconut from plumule explants / Luis Sáenz, José Luis Chan, María Narvaez, and Carlos Oropeza -- Micropropagation of yucca species / Yessica López-Ramírez, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma. de Lourdes de la Rosa-Carrillo, and Eugenio Pérez-Molphe-Balch -- Auxin immunolocalization in Coffea canephora tissues / Ruth E. Márquez-López, Ángela Ku-González, Hugo A. Méndez-Hernández, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas -- Somatic embryogenesis in common bean Phaseolus vulgaris L. / José Luis Cabrera-Ponce, Itzel Anayetzi González-Gómez, Claudia G. León-Ramírez, José A. Sánchez-Arreguín, and Alba E. Jofre y Garfias -- Induction of somatic embryogenesis in Jatropha curcas / Rosa M. Galaz-Ávalos, Heydi G. Martínez-Sánchez, and Víctor M. Loyola-Vargas -- In vitro proliferation of female buds for induction of somatic embryogenesis from false horn Plantain (AAB, cv. Curraré) / Rosa Maria Escobedo-Gracia-Medrano, Carlos Iván Cruz-Cárdenas, Lucila Aurelia Sánchez-Cach, José Roberto Ku-Cauich, and Wilma Aracely González-Kantún -- Somatic embryogenesis in Theobroma cacao L. / Claudia Garcia, Jean-Philippe Marelli, Juan Carlos Motamayor, and Cristiano Villela -- Somatic embryogenesis of Quercus suber L. from immature zygotic wmbryos / Pilar S. Testillano, Aránzazu Gómez-Garay, Beatriz Pintos, and María C. Risueño -- Cryotherapy : a novel method for virus eradication in economically important plant species / Min-Rui Wang, Long Chen, Zhibo Zhang, Dag-Ragnar Blystad, and Qiao-Chun Wang -- Cryopreservation of pineapple shoot tips by the droplet vitrification technique / Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ergun Kaya, Lívia de Jesus Vieira, and Ronilze Leite da Silva -- Cryopreservation of pollen grains of pineapple and other bromeliads / Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, and Ronilze Leite da Silva -- Application of in Casa pollination and embryo rescue techniques for breeding of agave species / Benjamín Rodríguez-Garay, Sigifredo López-Díaz, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, and Ernesto Tapia-Campos -- Haploid and doubled haploid plant production in carrot using induced parthenogenesis and ovule excision in vitro / Agnieszka Kiełkowska, Adela Adamus, and Rafal Baranski -- Using flow cytometry analysis in plant tissue culture derived plants / Rosa María Escobedo-Gracia-Medrano, Martha Josefa Burgos-Tan, José Roberto Ku-Cauich, and Adriana Quiroz-Moreno -- Procedure for estimating the tolerance and accumulation of heavy metals using plant cell cultures / Antonio Bernabé-Antonio, Amalia Maldonado-Magaña, María Elena Estrada-Zúñiga, Leticia Buendía-González, and Francisco Cruz-Sosa -- Proteomics as a tool to study molecular changes during plant morphogenesis in vitro / André Luis Wendt dos Santos, Ricardo Souza Reis, Angelo Schuabb Heringer, Eny Iochevet Segal Floh, Claudete Santa-Catarina, and Vanildo Silveira -- Proteomic analysis of non-model plant tissues using phenol extraction, two-dimensional electrophoresis, and MALDI mass spectrometry / Petra Peharec Štefanić, Mario Cindrić, and Biljana Balen -- Chromatin immunoprecipitation (ChiP) protocol for the analysis of gene regulation by histone modifications in Agave angustifolia haw / Rosa Us-Camas and Clelia De-la-Peña -- Transcription factors : their role in the regulation of somatic embryogenesis in Theobroma cacao L. and other species / Claudia Garcia, Dahyana Britto, and Jean-Philippe Marelli -- MicroRNA expression and regulation during maize somatic embryogenesis / Brenda Anabel López-Ruiz, Vasti Thamara Juárez-González, Elva Carolina Chávez-Hernández, and Tzvetanka D. Dinkova -- Elaboration of transcriptome during the induction of somatic embryogenesis / Elsa Góngora-Castillo, Geovanny I. Nic-Can, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas -- Induction of specialized metabolism in in vitro cultures of Capsicum chinense Jacq / Felipe A. Vázquez-Flota and María de Lourdes Miranda-Ham -- Analysis of terpenoid indole alkaloids, carotenoids, phytosterols, and NMR-based metabolomics for Catharanthus roseus cell suspension cultures / Mohd Zuwairi Saiman, Natali Rianika Mustafa, and Robert Verpoorte -- Transformed root culture : from genetic transformation to NMR-based metabolomics / Andrey S. Marchev, Zhenya P. Yordanova, and Milen I. Georgiev -- Genetic transformation of Pentalinon andrieuxii tissue cultures / Yeseña Burgos-May, Elidé Avilés-Berzunza, Luis Manuel Peña-Rodríguez, and Gregorio Godoy-Hernández -- Appendix A : Components of the culture media
Summary This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Cell Culture Protocols, Fourth Edition aims to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture
Bibliography Includes bibliographical references and index
Subject Plant cell culture -- Laboratory manuals
Cell culture.
Cell Culture Techniques
Plant Cells
Cells, Cultured
Botany & plant sciences.
Science -- Life Sciences -- Botany.
Cell culture
Plant cell culture
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Loyola-Vargas, Victor M., editor
Ochoa-Alejo, Neftalí, editor
ISBN 9781493985944
1493985949
1493985930
9781493985937