| Description |
1 online resource (xi, 399 pages) : illustrations (some color) |
| Series |
Springer Protocols |
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Methods in molecular biology, 1064-3745 ; 559 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 559.
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| Contents |
Analysing caspase activation and caspase activity in apoptotic cells / S. Kumar and L. Dorstyn -- Flow cytometry-based apoptosis detection / D. Wlodkowic, J. Skommer and Z. Darzynkiewicz -- Live to dead cell imaging / S.W. Tait [and others] -- Detection of apoptosis in tissue sections / E. Csizmadia and V. Csizmadia -- Detection of apoptosis in cell-free systems / D. Chandra and D.G. Tang -- Methods to analyze cellular necroptosis / B. Miao and A. Degterev -- Detection of cell death by autophagy / N. Gurusamy and D.K. Das -- Methods to analyze transglutamination of proteins involved in apoptosis / Z. Nemes and L. Fesus -- Methods to analyze S-nitrosylation of proteins involved in apoptosis / N. Azad, A.K. Iyer and Y. Rojanasakul -- Application of in vivo EPR for tissue pO2 and redox measurements / N. Khan and D.K. Das -- Assays to measure p53-dependent and -independent apoptosis / D.C. Phillips [and others] -- Measurement of changes in Cdk2 and cyclin o-associated kinase activity in apoptosis / R. Roset and G. Gil-Gomez -- Fluorometric methods for detection of mitochondrial membrane permeabilization in apoptosis / S.S. Roy and G. Hajnoczky -- Regulation of apoptosis by the unfolded protein response / A. Fribley, K. Zhang and R.J. Kaufman -- Detection of uncoupling protein-2 (UCP2) as a mitochondrial modulator of apoptosis / Z. Derdak, T.A. Garcia and G. Baffy -- Multiple approach to analyzing the role of microRNAs in apoptosis / R. Spizzo and G.A. Calin -- Assessment of apoptotic cell phagocytosis by macrophages / K.A. McPhillips and L.P. Erwig -- Detection of apoptosis in mammalian development / L. Lin [and others] -- Detection of apoptosis in the central nervous system / Y. Lee and P.J. McKinnon -- Genetic mapping of anti-apoptosis pathways in myeloid progenitor cells / D. Liu and Z. Songyang -- Analysis of apoptosis in isolated primary cardiac myocytes / A. Mandl, A. Toth and P. Erhardt -- Cell death in myoblasts and muscles / L.M. Schwartz [and others] -- Reliable method for detection of programmed cell death in yeast / X. Teng and J.M. Hardwick -- Detection of cell death in Drosophila / K. McCall, J.S. Peterson and T.L. Pritchett -- Detecting apoptotic cells and monitoring their clearance in the nematode Caenorhabditis elegans / N. Lu [and others] -- Detection of herpes simplex virus dependent apoptosis / C.R. Cotter and J.A. Blaho |
| Summary |
The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Apoptosis -- Laboratory manuals
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Molecular biology -- Laboratory manuals
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DNA Damage -- physiology
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Molecular biology.
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Apoptosis -- physiology.
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DNA Damage -- physiology.
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Apoptosis.
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Apoptosis
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Molecular biology
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Erhardt, Peter
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|
Toth, Ambrus
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| LC no. |
2009931045 |
| ISBN |
9781603270168 |
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1603270167 |
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9781603270175 |
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1603270175 |
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9781607615101 |
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160761510X |
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9781617794896 |
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1617794899 |
|
