Title Clinical applications of capillary electrophoresis : methods and protocols / edited by Terry M. Phillips, Heather Kalish

Published New York : Humana Press, ©2013

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Description 1 online resource (xiii, 311 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; 919 Methods in molecular biology (Clifton, N.J.) ; 919. 1064-3745 Contents Overview of CE in clinical analysis / David S. Hage -- Monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection / Petr Tu°ma and Karel Štulík -- Selective determination of sulfates, sulfonates, and phosphates in urine by capillary electrophoresis/mass spectrometry / Svenja-Catharina Bunz and Christian Neusüss -- Analysis of neurotransmitter amino acids by CE-LIF detection in biological fluids / Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru -- Expanded newborn screening of inborn errors of metabolism by capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) / Philip Britz-McKibbin -- Development of an on-site measurement system for salivary stress-related substances based on microchip CE / Yoshihide Tanaka and Nahoko Naruishi -- Simultaneous determination of atenolol and amiloride by capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) / Khaldun M. AL Azzam and Hassan Y. Aboul-Enein -- Homogeneous immunoassay of thyroxine based on microchip electrophoresis and chemiluminescence detection / Shulin Zhao and Yi-Ming Liu -- High-throughput profiling of the serum n-glycome on capillary electrophoresis microfluidics systems / Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert -- Screening of matrix metalloproteinase inhibitors by microanalysis with fluorescence detection / Xin Hai, Erwin Adams, and Ann Van Schepdael -- ABO genotyping by capillary electrophoresis / James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai -- Separation of hemoglobin variants by capillary electrophoresis / Frédéric Cotton and Béatrice Gulbis -- Newborn screening for hemoglobinopathies using capillary electrophoresis / P.C. Giordano -- Application of CZE in the differentiation of Staphylococcus aureus strains / Bogusław Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz -- Multiplex and quantitative pathogen detection with high-resolution capillary electrophoresis-based single-strand conformation polymorphism / Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung -- Application of CGE to virus identification / Julia A. Fruetel and Victoria A. VanderNoot -- Capillary electrophoresis-based proteomic techniques for biomarker discovery / Xueping Fang, Chenchen Wang, and Cheng S. Lee -- Integrated affinity and electrophoresis systems for multiplexed biomarker analysis / Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley -- Urinary proteomics based on capillary electrophoresis coupled to mass spectrometry in kidney disease / Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zürbig -- Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-acid glycoprotein isoforms as an atherothrombosis biomarker / Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa, and Mercedes de Frutos -- Chip-based immunoassays / Akwasi A. Apori and Amy E. Herr -- CE analysis of g-globulin mobility and potential clinical utility / Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe -- Cytokine analysis by immunoaffinity capillary electrophoresis / Mark Mendonca and Heather Kalish -- Capillary electrophoresis for the detection of fragile X expanded alleles / Rong Mao, Pinar Bayrak-Toydemir, and Elaine Lyon -- Analysis of microsatellite instability by microfluidic-based electrophoresis / Natalia Elfimova, Wafa Amer, and Margarete Odenthal -- HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease / Ewa H. Lavant and Joyce Carlson Summary Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used. Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science. Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research Analysis Life sciences Biochemistry Electrophoresis capillaire elektroforese capillary electrophoresis klinische onderzoeken clinical investigations sulfaten sulfates sulfonaten sulfonates fosfaten phosphates aminozuren amino acids chemiluminescence immunoassays Analytical Chemistry Analytische chemie Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (Springer Protocols, viewed Oct. 29, 2012) Subject Capillary electrophoresis -- Laboratory manuals Electrophoresis, Capillary Capillary electrophoresis Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Phillips, Terry M. Kalish, Heather. ISBN 9781627030298 1627030298 162703028X 9781627030281

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