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Author
Westermeier, Reiner.
Title
Electrophoresis in practice : a guide to methods and applications of DNA and protein separations / Reiner Westermeier, in collaboration with Sonja Gronau ... [and others]
Edition
Third edition
Published
Weinheim ; Chichester : Wiley-VCH, [2001]
©2001
Copies
Location
Call no.
Vol.
Availability
W'BOOL
547.704572 Wes/Eip 2001
AVAILABLE
Description
xviii, 349 pages : illustrations (some color) ; 25 cm + 1 folded template
Contents
Pt. I. Fundamentals -- 1. Electrophoresis -- 2. Isotachophoresis -- 3. Isoelectric focusing -- 4. Blotting -- 5. Interpretation of electropherograms -- 6. Proteome Analysis -- 7. Instrumentation -- Pt. II. Methods -- Method 1. PAGE of dyes -- Method 2. Agarose and immuno electrophoresis -- Method 3. Titration curve analysis -- Method 4. Native PAGE in amphoteric buffers -- Method 5. Agarose IEF -- Method 6. PAGIEF in rehydrated gels -- Method 7. Horizontal SDS-PAGE -- Method 8. Vertical PAGE -- Method 9. Semi-dry blotting of proteins -- Method 10. IEF in immobilized pH gradients -- Method 11. High-resolution 2D electrophoresis -- Method 12. PAGE of double stranded DNA -- Method 13. Native PAGE of single stranded DNA -- Method 14. Denaturing gradient gel electrophoresis -- Method 15. Denaturing PAGE of DNA
Notes
Previous ed.: Weinheim : VCH, 1997
Bibliography
Includes bibliographical references (pages [333]-340) and index
Subject
DNA -- Separation -- Laboratory manuals.
Electrophoresis -- Laboratory manuals.
Proteins -- Separation -- Laboratory manuals.
Genre/Form
Laboratory manuals.
Author
Barnes, Neil.
LC no.
2001268000
ISBN
3527303006
Other Titles
Elektrophorese-Praktikum. English
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