Title The eukaryotic RNA exosome : methods and protocols / edited by John LaCava, Štěpánka Vaňáčová

Published New York : Humana Press, [2020]

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Description 1 online resource (1 PDF file (xiii, 517 pages)) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; volume 2062 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2062. 1064-3745 Springer protocols (Series) 1949-2448 Summary This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes' origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction of networks, and establish critical structure-function relationships. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This book begins with an introduction to RNA exosome biomedical relevance (Chapter 1) and then steps back to examine the origins of exosome activity in bacteria (Chapters 2-3) and archaea (Chapter 4). From there, methods of studying exosome RNA substrates are covered (Chapters 5-10), followed by the study of exosome adapter complexes and the broader interaction networks that impart selectivity to pathways the exosome participates in (Chapters 11-16). Finally, structural and mechanistic biochemical studies are covered, with emphasis on methods that use recombinant expression and exogenous reconstitution (Chapters 17-24) Bibliography Includes bibliographical references and index Notes Online resource; title from PDF title page (SpringerLink, viewed December 17, 2019) Subject Eukaryotic cells. RNA. Exosome Multienzyme Ribonuclease Complex RNA -- metabolism Eukaryotic Cells RNA Eukaryotic cells RNA Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author LaCava, John, editor Vaňáčová, Štěpánka, editor ISBN 9781493998227 1493998226

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