| Description |
1 online resource |
| Contents |
Preface to the Third Edition; Preface to the First Edition; Abbreviations; List of color figures ; PART I: PLANT TISSUE CULTURE; INTRODUCTION; New technologies; Origin of biotechnology; History; LABORATORY ORGANIZATION; Washing facility; General laboratory and media preparation area; Transfer area; Culturing facilities; Green houses; Laboratory and personal safety; NUTRITION MEDIUM; Units for solution preparation; Media composition; STERILIZATION TECHNIQUES; Preparation of sterile media, |
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Containers and small instruments; Maintenance of aseptic conditions; Sterilization of explant; Protocols; TYPES OF CULTURE; Cytodifferentiation; Organogenic differentiation; Types of culture; Protocols; MICROPROPAGATION; Axillary bud proliferation approach; Organogenesis; Embryogenesis; Advantages of micropropagation; Problems associated with micropropagation; Protocols; CELL SUSPENSION AND SECONDARY METABOLITES; Types of suspension cultures; Growth measurements; Synchronization of suspension culture cells; Technique for single cell culture--Bergmann cell plating technique; Production of secondary metabolites; Morphological and chemical differentiation; Application; Problems associated with secondary metabolite production; Immobilized cell systems; Biotransformation; Secondary metabolite production using genetically engineered plant cell cultures; Protocols; IN VITRO PRODUCTION OF |
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HAPLOIDS; Androgenic methods; Significance and uses of haploids; Problems; Gynogenic haploids; Chromosome elimination technique for production of haploids in cereals (Barley and wheat); Protocol; PROTOPLAST ISOLATION AND FUSION; Protoplast isolation; Protoplast development; Cell wall formation; Growth, |
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Division and plant regeneration; Somatic hybridization; Identification and selection of hybrid cells; Verification & characterization of somatic hybrids; Chromosome number in somatic hybrids; Cybrids; Potential of somatic hybridization; Problems and limitations of somatic hybridization; Protocol; SOMACLONAL VARIATION; Nomenclature; Schemes for obtaining somaclonal variation; Application of somaclonal variation; Basis of somaclonal variation; Disadvantages; Gametoclonal variation; GERMPLASM STORAGE & CRYOPRESERVATION; Cryopreservation; Slow growth method; Achievements; Applications; Limitations; ; PART II: GENETIC MATERIAL AND ITS ORGANIZATION; GENETIC MATERIAL; Sugars; Amino acids; Nucleotides; Polynucleotides; Genetic material; ORGANIZATION OF DNA AND GENE EXPRESSION; Different forms of DNA; Supercoiling--Tertiary structure of DNA; Cruciform--Tertiary structure of DNA; Eukaryotic |
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DNA organization into nucleosomes; DNA content; Denaturation; Flow of genetic information: central dogma; Organization of genes in DNA molecules; Plant gene structure as discontinuous gene; Control sequences; Types of RNA molecules; Transcription; Genetic code and translation; ; PART III: RECOMBINANT DNA TECHNOLOGY; BASIC TECHNIQUES; Agarose gel electrophoresis; Pulsed field gel electrophoresis; Polyacrylamide gel electrophoresis; Nucleic acid blotting; Protein blotting; Dot blot technique; Autoradiography; E. coli transformation; Protocol; GENE CLONING: Cutting and joining DNA Molecules; Introduction; Enzymes for cutting: Restriction endonucleases; Joining DNA molecules; DNA modifying enzymes; Linkers and adaptors; Protocol; GENE CLONING: Vectors; Cloning vectors--features; Biology of E |
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Coli K-12; Plasmids; Cosmids; Bacteriophage vectors; Phagemids; Yeast artificial chromosome (YAC); Bacterial artificial chromosome (BAC); P1 phage vectors; P1 derived artificial chromosome (PAC); Transposon; Shuttle vectors; Expression vectors; Protocols; GENE CLONING: cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences; Gene libraries; cDNA cloning; Genomic cloning; Identification and analysis of cloned genes; Approaches for identification of genes; Detection of nucleic acid sequences; Radioactive probe preparation and labeling; Non-radioactive labeling; DNA sequencing; POLYMERASE CHAIN REACTION; Introduction; Procedure; Components in a polymerase chain reaction; Inverse PCR; Reverse transcriptase mediated PCR (RT-PCR); Cloning of PCR products; Applications; Advantages; Problems; Protocol; IN VITRO MUTAGENESIS; Site directed mutagenesis; Insertional |
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Mutagenesis; TRANSPOSON GENETIC ELEMENTS AND GENE TAGGING; Transposable elements in bacteria; Transposable elements in eukaryotes; Classification; Transposon gene tagging; GENE ISOLATION ; Chromosome maps; General strategies for cloning genes from plants; MOLECULAR MARKERS AND MARKER-ASSISTED SELECTION; Morphological markers; Biochemical markers; Molecular markers; History of evolution of molecular markers; Non-PCR based approaches; PCR based techniques; Targeted PCR and sequencing; Single Nucleotide Polymorphism (SNP); Molecular markers and plant diversity; Fingerprinting; Marker assisted selection; Protocol; GENE TRANSFER IN PLANTS; Transient and stable expression; Marker genes; Chimeric gene vectors; Gene transfer methods; Status and expression of transferred genes; Protocols; CHLOROPLAST AND MITOCHONDRION DNA TRANSFORMATION; Chloroplast Transformation; The Plastid |
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Chromosome; Selectable markers; Homoplastomic lines development; Advantages; Applications; Limitations of Chloroplast Transformation; Mitochondrion transformation; Targetting of Transgene Product into Chloroplasts and Mitochondria; TRANSGENICS IN CROP IMPROVEMENT ; Resistance to biotic stresses; IMPACT OF RECOMBINANT DNA TECHNOLOGY ; Agriculture; Status of transgenic crops; Benefits of transgenic crops; Biotech crops in India; Insect resistant plants through transgenic approach; Herbicide tolerant plants through transgenic approach; Plant based pharmaceuticals; Production of biofuels; Transgenic crops in future; Biotechnology in Process Industry; Bioremediation; Healthcare applications; Future; BIOSAFETY CONCERNS AND REGULATORY FRAMEWORK; Risk analysis; Containment; Biosafety levels; Good laboratory practices; Good laboratory practices; Risk Management in Field Trials; National Biosafety Regulatory |
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Framework in India; Recombinant DNA safety Guidelines; Revised Guidelines for Research in Transgenic Plants; Cross border movement of transgenic germplasm for research purposes; Biosafety concerns; Socio-economic and ethical considerations; International protocols and conventions on biosafety; Regulatory frameworks in different countries; GENOMICS; Introduction; Mapping of prokaryotic genomes; Mapping of eukaryotic genomes; Gene location in DNA sequences; Yeast (S |
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Cerevisiae) genome; Human genome; Arabidopsis genome; Rice genome; Functional genomics; Patterns of gene expression; Proteomics; Metabolomics; Phenomics; BIOINFORMATICS; Development of Bioinformatics; Role of Internet in Bioinformatics; Online; Browsing working and downloading; Sequences and Nomenclature; File Formats; Files of Structural data; Submission of Sequences; Databases; Molecular Biology Databases; Bioinformatics Databases and analysis Services; Specialized Resources; Bioinformatics Databases and Analysis Services; Bioinformatics tools for access to databases; Integrated Molecular Biology Database system; Database integration strategies; Detection of genes; Applications; Challenges> |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (EBSCO, viewed December 19, 2018) |
| Subject |
Plant biotechnology.
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TECHNOLOGY & ENGINEERING -- Agriculture -- General.
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Plant biotechnology.
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| Form |
Electronic book
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| ISBN |
9781315275369 |
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1315275368 |
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9781439843604 |
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1439843600 |
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