| Description |
1 online resource (xi, 290 pages) : illustrations (some color) |
| Series |
Springer protocols handbooks, 1949-2448 |
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Springer protocols (Series) 1949-2448
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| Contents |
Isolation and identification of Yersinia pestis / Zhizhen Qi, Qingwen Zhang, Yong Jin, Youquan Xin, Haihong Zhao, Xiaoyan Yang, Xiaoqing Xu, and Xuefei Zhang -- Isolation and characterization of Yersinia pestis bacteriophages / Xiangna Zhao -- Extraction of chromosomal DNA and plasmid from Yersinia pestis / Yazhou Zhou -- Extraction of total RNA from Yersinia pestis / Haili Wang and Yanping Han -- Genotyping of Yersinia pestis / Yanjun Li and Yujun Cui -- Genome-wide variation analysis of Yersinia pestis / Chao Yang and Yujun Cui -- Genome-wide detection of expressed sRNAs in Yersinia pestis with RNA-seq / Yanping Han -- Validation of Yersinia pestis sRNAs with nonradioactive-labeled northern blotting / Yanping Han -- Determination of transcription start sites (TSSs) in Yersinia pestis with a primer extension assay / Yanping Han -- Purification of Hfq-associated RNAs with RNA immunoprecipitation (RIP) / Yanping Han -- Overexpression of target sRNAs in tightly controlled multicopy plasmids / Xiaofang Gao, Zizhong Liu, and Yanping Han -- Target validation of sRNA with a GFP reporter gene fusion system / Xiaofang Gao, Zizhong Liu, and Yanping Han -- Inducible gene expression in Yersinia pestis / Juping Zhao and Yicheng Sun -- Analysis of yops secretion by Yersinia pestis type III secretion system / Zongmin Du and Shiyang Cao -- Co-immunoprecipitation analysis for the detection of protein-protein interactions in Yersinia pestis / Shiyang Cao and Zongmin Du -- Detection of type III secretion system needle assembly in Yersinia pestis by cross-linking YscF / Shiyang Cao and Zongmin Du -- Evaluation of protein translocation into eukaryotic cells by Yersinia pestis / Yafang Tan -- Adhesion analysis of Yersinia pestis to host cells / Yafang Tan -- Construction of gene deletion mutants in Yersinia pestis / Wenliang Li, Dan Rong, and Yanping Han -- CRISPR-Cas12a-assisted recombineering in Yersinia pestis / Juping Zhao and Yicheng Sun -- Plasmid curing in Yersinia pestis / Yazhou Zhou -- Genetic regulation analysis of Yersinia pestis / Yiquan Zhang -- Investigation of biofilm formation in Yersinia pestis / Haihong Fang -- Extraction and purification of F1 capsule antigen from Y. pestis / Xiaoyi Wang -- Screening of regulator responses to Yersinia pestis infection with a concatenated tandem array of transcription factor response element (catTFRE) pulldown / Chune Jing -- Animal models for Yersinia pestis / Yujing Bi and Xiaoyi Wang -- Antibiotic resistance evaluation of Yersinia pestis / Jingyu Guo -- PCR for detection and identification of Yersinia pestis / Yong Zhao -- Immunological methods for detection and identification of Yersinia pestis / Yong Zhao -- Analysis of fatty acids in Yersinia pestis by gas chromatography / Yafang Tan -- Evaluation of efficacy of Yersinia pestis vaccines / Xiaoyi Wang -- Online resources for Yersinia pestis / Yanfeng Yan -- Effects of Y. pestis on macrophages / Yujing Bi |
| Summary |
This protocol book includes common and advanced research protocols for Yersinia pestis research and for its identification, genotyping and source-tracing. This book includes protocols for genomic and transcriptomic analysis, small RNA study, protein-protein interaction, gene deletion. It will be helpful for researchers in this field, graduate students, clinicians, and researchers in infectious diseases, systems biology, and evolution etc |
| Bibliography |
Includes bibliographical references |
| Notes |
Online resource; title from PDF title page (SpringerProtocols, viewed June 5, 2018) |
| Subject |
Yersinia pestis -- Laboratory manuals
|
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Yersinia pestis -- isolation & purification
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Yersinia pestis -- genetics
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Microbiology (non-medical)
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Science -- Life Sciences -- Biology -- Microbiology.
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Yersinia pestis
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Yang, Rui-fu
|
| ISBN |
9789811079474 |
|
9811079471 |
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