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E-book

Title Laser capture microdissection : methods and protocols / edited by Graeme I. Murray
Edition Third edition
Published New York : Humana Press : Springer, ©2018

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Description 1 online resource (xv, 412 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; v. 1723
Methods in molecular biology (Clifton, N.J.) ; v. 1723. 1064-3745
Contents Laser capture microdissection : insights into methods and applications / Meera Mahalingam -- Laser microdissection-based microproteomics of formalin-fixed and paraffin-embedded (FFPE) tissues / Remi Longuespee, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw -- Laser microdissection workflow for isolating nucleic acids from fixed and frozen tissue samples / Yelena G. Golubeva and Andrew C. Warner -- Protocol for the analysis of laser capture microdissected fresh-frozen tissue homogenates by silver-stained 1D SDS-PAGE / DaRue A. Prieto, Gordon Whitely, Donald J. Johann Jr., and Josip Blonder -- Next-generation sequencing analysis of laser-microdissected formalin-fixed and paraffin-embedded (FFPE) tissue specimens / Lavinia M€agel, Stephan Bartels, and Ulrich Lehmann -- Adaptation of laser microdissection technique to nanostring RNA analysis in the study of a spontaneous metastatic mammary carcinoma mouse model / Nadia P. Castro and Yelena G. Golubeva -- Laser capture microdissection as a tool to study the mucosal immune response in celiac disease / Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella -- Laser capture microdissection and isolation of high-quality RNA from frozen endometrial tissue / Michele Cummings, Georgia Mappa, and Nicolas M. Orsi -- Laser microdissection for human papillomavirus (HPV) genotyping attribution and tethylation pattern analyses of squamous intraepithelial lesions / Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall -- Laser capture microdissection and transcriptional analysis of sub-populations of the osteoblast lineage from undecalcified bone / Efrain Pacheco, Rong Hu, and Scott Taylor -- Cell type-specific laser capture microdissection for gene expression profiling in the human brain / Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag -- Isolation of pure populations of neurons by laser capture microdissection : methods and application in neuroscience / Renee Morris and Prachi Mehta -- Laser capture microdissection in traumatic brain injury research : obtaining hippocampal subregions and pools of injured neurons for genomic analyses / Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich -- Isolation of distinct types of neurons from fresh brain tissue using laser microdissection in combination with high-performance liquid chromatography-mass spectrometry / Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May -- Immuno-guided laser-capture microdissection of glial cells for mRNA analysis / Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud -- Immuno-laser-capture microdissection for the isolation of enriched glial populations from frozen post-mortem human brain / Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath -- Laser-capture microdissection for the analysis of rat and human spinal cord ependyma by qPCR / Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado -- Isolation of amyloid plaques and neurofibrillary tangles from archived alzheimer's disease tissue using laser-capture microdissection for downstream proteomics / Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski -- Cell-specific RNA quantification in human SN DA neurons from heterogeneous post-mortem midbrain samples by UV-laser microdissection and RT-qPCR / Johanna Duda, Michael Fauler, Jan Gr€undemann, and Birgit Liss -- Laser-capture microdissection for layer-specific analysis of enteric ganglia / Corinna Rosenbaum, Martina Bottner, Thilo Wedel, and Marco Metzger -- Laser microdissection-liquid chromatography-tandem mass spectrometry workflow for post-mortem analysis of brain tissue / David C. Hondius, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, Ka Wan Li, and August B. Smit -- Laser-capture microdissection and RNA extraction from perfusion-fixed cartilage and bone tissue from mice implanted with human iPSC-derived MSCs in a calvarial defect model / Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, and Joel S. Pachter -- Laser-capture microdissection-based RNA-Seq of barley grain tissues / Ronny Brandt, Martin Mascher, and Johannes Thiel
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed January 18, 2018)
Subject Molecular biology -- Methodology
Microdissection.
Lasers.
Genetics -- Technique.
Lasers
Microdissection -- methods
Genetic Techniques
lasers (optical instruments)
Biotechnology.
Science -- Biotechnology.
Lasers
Microdissection
Molecular biology -- Methodology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Murray, Graeme I
ISBN 9781493975587
1493975587
9781493975570
1493975579