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Title Microbiomics dimensions, applications, and translational implications of human and environmental microbiome research / edited by Manousos E. Kambouris and Aristea Velegraki
Published London : Academic Press, 2020
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Description 1 online resource
Series Translational and applied genomics
Translational and applied genomics series.
Contents Front Cover -- Microbiomics -- Copyright Page -- Contents -- List of Contributors -- 1 Introduction: The Microbiome as a Concept: Vogue or Necessity? -- References -- I. Classes and Kinds of Microbiomes -- 2 Bacteriome and Archaeome: The Core Family Under the Microbiomic Roof -- Introduction -- DNA Exchange -- The Pioneers, Their Vision and Their Means -- Diversity -- Habitats, Settings, and Formats -- Environmental Adaptability, Monitoring, and Engineering -- The (Near-Term) Way Ahead -- References -- 3 Myc(et)obiome: The Big Uncle in the Family -- Introduction -- Emergence and Establishment
Definition and Identity -- Mycobiome: Status, Categories, and Essence -- Mycobiome: Structure and Composition -- Studying the Mycobiome -- Microscopy -- Culture and Culturomics -- Immunoassays -- Metagenomics -- Select Mycobiomic Research: Some Working Examples -- Human Mycobiomes -- Remote Effects, Communication, and Control Functions of Mycobiomes -- Gut-Brain Axis and the Mycobiome Factor -- Mycorrhizal Databuses -- References -- 4 Virome: The Prodigious Little Cousin of the Family -- Introduction -- The Viral Components of the Microbiome -- The Environmental Virome -- The Plant Virome
Introduction-The Romantic Past -- The Modern Pedigree -- Metamodernism: The Changing Environment -- Metamodernism: The Methods -- Mass Spectrometry -- Immunoassays -- Genomics -- Microscopy -- Conclusion: A Peek of the Future -- References -- 6 Panmicrobial Microarrays -- Introduction -- Invention, Definition, and Rationale of Microarrays -- Pedigree and Categories of Microarrays -- Comparison to the State of the Art -- Trade-Offs and Prospects -- Methodology -- Development and Optimization -- Types of Labeling Signal -- Amplification -- The Microbiomic Aspect of Microarray Concepts
The Genomic Aspect -- Phenotypic Microarrays -- Live Cell Microarrays -- Conclusion -- References -- 7 Metagenomics in Microbiomic Studies -- Introduction -- Commensals and Infectious Agents -- Five Key Metrics -- Sensitivity -- Efficiency -- Bias/Universality -- Taxonomic Classification of Novel Microbes -- Contamination -- Total DNA or RNA Sequencing Using Illumina -- Specimen Collection and Storage -- DNA/RNA Extraction -- Isolation of Relevant DNA/RNA -- Library Preparation -- Sequencing -- Alignment -- Tabulation -- Ribosomal RNA Genes rrs and rrl -- Conserved Sequences
The Human Virome -- Methodological Challenges Associated With Virome Studies -- The Host-Virus Interactome -- Network Analysis: A Roadmap to Explore Host-Virus Interactions -- Prospecting the Continuum of Interactions Within the Virosphere -- The Double-Stranded DNA Virus-Host Interactome -- Humans, Apes, and Monkeys -- Dolphins -- Bats -- Birds -- Amoebas -- Fishes -- Bacteria -- Future Perspectives, Aspects, and Prospects -- References -- II. The Study of Microbiota and Microbiomes -- 5 Identifying Microbiota: Genomic, Mass-Spectrometric, and Serodiagnostic Approaches
Notes Includes index
Subject Medical microbiology.
Medical microbiology.
Form Electronic book
Author Kambouris, Manousos E.
Velegraki, Aristea.
ISBN 0128172827
9780128172827