| Description |
1 online resource |
| Contents |
Cover -- Half Title -- Title Page -- Copyright Page -- Dedication -- Preface -- Table of Contents -- About the Authors -- Part A: Theory and Basic Techniques -- 1. Introduction -- 2. Microscopy -- 2.1 Light Microscopy -- 2.2 Electron Microscopy -- 3. Botanical Microtechnique and Microtomy -- 3.1 Killing and Fixation -- 3.2 Dehydration and Infiltration -- 3.3 Embedding and Sectioning -- 4. Stains (Dyes) -- 4.1 Natural Dyes -- 4.2 Coal-Tar Dyes -- 4.3 Other Substances Acting as Stains -- 5. Staining Techniques -- 5.1 Types of Staining -- 5.2 Differentiation -- 5.3 Mordants |
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5.4 Clearing (Bleaching) -- 5.5 Staining Schedules -- 6. Centrifugation -- 6.1 Basic Principle of Sedimentation -- 6.2 Instrumentation -- 6.3 Mechanism of Centrifugation -- 6.4 Sedimentation Co-efficient -- 6.5 Classification of Centrifuges and their Uses -- 7. Colorimetry and Spectrophotometry -- 7.1 Colorimetry -- 7.2 Spectrophotometry -- 8. Common Adhesives and Mounting Media -- 8.1 Common Adhesives -- 8.2 Mounting Media -- 8.3 Substitute for Immersion Oil in Microscopy -- 9. Plant Collection and Herbarium Techniques -- 9.1 Plant Collection and Preservation -- 9.2 Herbarium |
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10. Biostatistics -- 10.1 Basic Statistics (Descriptive Statistics) -- 10.2 Analysis of Variance (ANOVA -- Components of Mean) -- 10.3 Null Hypothesis -- 10.4 T-test -- 10.5 Coefficient of Variation -- 10.6 Heritability -- 10.7 Genetic Advance -- 10.8 Correlation -- 10.9 Path Analysis -- 10.10 Residual Effect -- Part B: Recent Advances in Plant Techniques -- 11. Histochemical Methods -- 11.1 Preparation of Sections for Histochemical Studies -- 11.2 Carbohydrates -- 11.3 Proteins -- 11.4 Detection of Ions in Plant/Animal Tissues -- 11.5 Nucleic Acids -- 11.6 Lignin, Suberin and Cutin |
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11.7 Cytokinins and Auxins -- 11.8 Latex and Rubber -- 12. Electrophoresis -- 12.1 Modes of Electrophoresis -- 12.2 Detection and Quantitative Assay -- 12.3 Discontinuous (Disc) Gel Electrophoresis -- 12.4 Protocols -- 13. Molecular Techniques -- 13.1 Genomic DNA Extraction -- 13.2 Quantification of DNA Samples Isolated -- 13.3 Quality of the DNA Samples Isolated -- 13.4 Agarose Gel Electrophoresis -- 13.5 Polymerase Chain Reaction -- 13.6 Types of PCR -- 13.7 Molecular Markers -- 13.8 Other Approaches -- 13.9 Protocols -- 14. Plant Tissue Culture -- 14.1 History |
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14.2 Setup a Tissue Culture Laboratory -- 14.3 Media Components and Preparation -- 14.4 Explant Preparation -- 14.5 Aseptic Technique -- 14.6 Pathways of Culture Cells and Tissues -- 14.7 Acclimatisation -- 14.8 Commercial Production -- 14.9 Culture Guide to Selected Plants -- 14.10 Meristem Cultures and Production of Disease Free Plants -- 14.11 Protoplast Fusion and Somatic Hybridisation -- 14.12 Anther and Pollen Culture -- 14.13 Micropropagation -- 14.14 Experimental Protocols -- 15. Chromatography -- 15.1 Paper Chromatography -- 15.2 Thin Layer (planar) Chromatography (TLC) |
| Summary |
This book deals with the basic concepts of Plant Science including botanical micro technique and microtomy, staining techniques, molecular techniques, plant tissue culture, electron microscopy, and cryopreservation and germplasm storage |
| Notes |
Machine-generated record |
| Genre/Form |
Electronic books
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| Form |
Electronic book
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| ISBN |
9781040106235 |
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1040106234 |
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