| Description |
1 online resource (xxi, 748 pages) : illustrations |
| Contents |
Intro -- Preface -- Acknowledgment -- Contents -- Editor and Contributors -- 1: Basic of Omics and Its Applications -- 1.1 Introduction -- 1.1.1 What Is Genome? -- 1.2 Genome to Genomics -- 1.2.1 DNA Sequencing -- 1.2.1.1 Sanger Sequencing -- 1.2.1.2 Next-Generation Sequencing -- Pyrosequencing -- Sequence by Synthesis -- Sequence by Ligation -- Ion Semiconductor Sequencing -- 1.3 Coverage -- 1.4 Genome Mapping -- 1.5 Proteomics -- 1.5.1 Amino Acids -- 1.5.2 Proteins -- 1.5.3 Why Proteomics? -- 1.5.4 How Do We Start Studying Proteomics? -- 1.5.4.1 Spot Detection -- 1.5.4.2 Fluorescence-Based Difference in Gel Electrophoresis (DIGE) -- 1.5.4.3 Identification -- 1.5.4.4 Mass Spectrometry -- 1.5.4.5 Separation -- 1.5.4.6 Activation -- 1.5.4.7 Mass Determination and Characterization -- 1.6 Transcriptomics -- 1.6.1 Expressed Sequence Tags (ESTs) -- 1.6.1.1 Serial Analysis of Gene Expression (SAGE) -- 1.6.1.2 Cap Analysis of Gene Expression (CAGE) -- 1.6.2 Microarray -- 1.6.3 RNA-Seq -- 1.7 Metabolomics -- 1.7.1 But What Are Metabolites? -- 1.7.2 Metabolome and Metabolic Reactions -- 1.7.3 But What Are the Analytical Techniques That We Need to Study Metabolomics? -- 1.7.4 Detection Methods -- 1.8 Lipidomics -- 1.8.1 Experimental Techniques -- 1.8.2 Lipid Extraction -- 1.8.3 Lipid Separation -- 1.8.4 Lipid Detection -- 1.8.5 Lipid Profiling -- Reference -- 2: An Introduction to Omics in Relevance to Industrial Microbiology -- 2.1 Introduction -- 2.2 Different Omics Techniques -- 2.2.1 Metagenomics -- 2.2.2 Cytomics -- 2.2.3 Metatranscriptomics -- 2.2.4 Metaproteomics -- 2.2.5 Metabolomics -- 2.2.6 Fluxomics -- 2.3 Advancement in Omics in Profiling and Characterization of Industrially Relevant Microbial Consortia -- 2.4 Sequential Workflow of Omics -- 2.5 Integrative Analysis of Omics Data -- 2.6 Omics Data Analysis Using Programming Language |
|
2.7 Applications of Omics in Industrial Microbiology -- 2.7.1 Application in Food Processing -- 2.7.2 Application in Dairy Industry -- 2.7.3 Application in Beverage Industry -- 2.7.4 Application in Pharmaceutical Industry -- 2.7.5 Application in Agricultural Biotechnology -- 2.8 Future Prospects and Limitations -- 2.9 Conclusion -- References -- 3: Databases and Tools for Microbial Genome and Human Microbiome Studies -- 3.1 Introduction -- 3.1.1 Prokaryotic Microbe -- 3.1.2 Eukaryotic Microbe -- 3.1.3 Acellular Microbe -- 3.2 Microbial Genome -- 3.3 History of Microbial Genome Sequencing -- 3.4 Introduction to Databases -- 3.5 Microbial Genome and Human Microbiome Databases -- 3.5.1 Global Genome Databases -- 3.5.2 Microbial Genome Database -- 3.5.3 Bacterial, Archaeal, and Viral Genomic Database -- 3.5.4 Species-Specific Genomic Database -- 3.5.5 Human Microbiome Databases -- 3.6 Bioinformatic Tools for Genomic Analysis -- 3.7 Conclusion -- References -- 4: CRISPR/Cas9 System: An Advanced Approach for the Improvement of Industrially Important Microorganisms -- 4.1 An Introduction to Industrial Microbiology -- 4.2 CRISPR/Cas System: An Introductory Overview -- 4.3 Classification of the CRISPR/Cas Systems -- 4.4 CRISPR/Cas9 System -- 4.5 Role of CRISPR/Cas9 in Improvement of Industrially Important Microorganisms -- 4.6 CRISPR/Cas9 Applications in Bacteria -- 4.7 CRISPR/Cas9 Applications in Yeasts -- 4.8 CRISPR/Cas9 Applications in Fungi -- 4.9 Applications of CRISPR/Cas9 in Microbes -- 4.10 Genome Editing -- 4.11 Transcriptional Control -- 4.12 CRISPR/Cas9 Optimization: Improvement of Editing Efficiency -- 4.12.1 Reduction of Off-Target Effects -- 4.12.1.1 Reduction of Off-Target Effects: sgRNA Design Approach -- 4.12.1.2 Reduction of Off-Target Effects: Modification in the Cas9 Protein -- 4.12.2 Reduction of Cas9 Toxicity Effects |
|
4.12.2.1 Reduction of Cas9 Toxicity: Regulation of the Cas9 Protein Expression -- 4.12.2.2 Reduction of Cas9 Toxicity: Exploitation of Endogenous CRCa System -- 4.12.3 Optimization of crRNA -- 4.12.3.1 SOMACA -- 4.12.3.2 Optimization of crRNA Length -- 4.12.4 Optimization of sgRNA -- 4.12.4.1 Optimization of the sgRNA Promoter -- 4.12.4.2 Optimization of the sgRNA Structure -- 4.12.5 Increase in Recombination Rates -- 4.13 Applications of CRISPR/Cas Systems in Gene Therapy -- 4.14 Delivery Methods -- 4.15 Conclusion -- References -- 5: Biomedical Application of Industrial Microbiology -- 5.1 Introduction -- 5.1.1 Basic Microbiology -- 5.1.2 Applied Microbiology -- 5.2 Products and Processes for Industrial Microbiology -- 5.3 Microbiology in Antibiotic Production -- 5.3.1 Fleming and the Discovery of the Antibiotic Penicillin -- 5.3.2 Commercial Production of Antibiotics -- 5.4 Recombinant DNA Technology (RDT) -- 5.5 Biopharmaceuticals -- 5.5.1 Enzymes -- 5.5.2 Vitamins and Amino Acids -- 5.5.3 Organic Acids -- 5.5.4 Biopolymers -- 5.6 Prebiotics and Probiotics -- 5.7 Vaccines and Immunizations -- 5.7.1 Types of Vaccines -- 5.7.1.1 Whole-Organism Vaccines -- 5.7.1.2 Subunit Macromolecules as Vaccines -- 5.7.1.3 DNA Vaccines -- 5.7.1.4 Recombinant Vector Vaccines -- 5.8 Clinical Use of Microbiology in the Detection and Therapy of Disease -- 5.8.1 Carcinogenicity Testing -- 5.8.2 Phage Therapy -- 5.8.3 Medical Devices -- 5.8.3.1 Biosensors -- 5.8.4 Yeast Two-Hybrid System (Y2H System) -- 5.9 Summary -- References -- 6: The Role of Whole-Genome Methods in the Industrial Production of Value-Added Compounds -- 6.1 Introduction -- 6.2 The Rise of Omics: Its Role in Industrial Biotechnology -- 6.3 Genomics -- 6.3.1 Genomics for Industrial Application and Production -- 6.3.2 Development of Microbial Strains -- 6.3.3 Fermentation and Post-fermentation Handling |
|
6.3.4 Viability of Strains and Their Compliance with Regulations -- 6.3.5 Safeguarding Inventions and Analyzing Products -- 6.4 Transcriptomics -- 6.4.1 Role of Transcriptomics in Industrial Microbiology -- 6.4.2 Studying Ethanol Tolerance in Microorganisms -- 6.4.3 To Assess Toxicity Sensitivity and Osmotic Stress Tolerance -- 6.4.4 Food Fermentation -- 6.5 Proteomics -- 6.5.1 Role of Proteomics in Industrial Microbiology -- 6.5.2 Lipid Biosynthesis in Microbes -- 6.5.3 Antifungal Production -- 6.5.4 Synthesis of Amino Acids -- 6.5.5 Production of Recombinant Proteins -- 6.5.6 Bio-mining -- 6.5.7 Studying Immobilized Cells in Biofilms -- 6.6 Metabolomics -- 6.6.1 Metabolomics and Its Role in Industrial Microbiology -- 6.6.2 Organic Acids -- 6.6.3 Enzyme Products -- 6.6.4 Biofuels -- 6.6.5 Antibiotics -- 6.7 Metagenomics -- 6.7.1 Industrial Importance -- 6.7.2 Industrial Enzymes -- 6.7.3 Antibiotics and Bioactive Compounds Obtained -- 6.7.4 Bioremediation Facilitated by Biosurfactant -- 6.7.5 Other Enzymes from Metagenome Source -- 6.8 Challenges in Omics for Industry -- 6.9 Sequencing Methods -- 6.9.1 First-Generation Sequencing -- 6.9.2 Chemical Degradation -- 6.9.3 Chain-Termination Method -- 6.9.4 Second-Generation Sequencing Methods -- 6.9.4.1 Roche 454 -- 6.9.4.2 Illumina -- 6.10 Third Generation of Sequencing Methods -- 6.10.1 True Single-Molecule Sequencing (tSMS) -- 6.10.2 Single-Molecule Real-Time Sequencing (SMRT) -- 6.10.3 Nanopore Sequencing -- 6.11 Annotation -- 6.12 Summary and Future Outlook -- References -- 7: New Developments in the Production and Recovery of Amino Acids, Vitamins, and Metabolites from Microbial Sources -- 7.1 Introduction -- 7.1.1 l-Methionine -- 7.1.1.1 Biosynthetic Pathway for Methionine Production -- 7.1.1.2 Methionine-Producing Microorganisms -- 7.1.1.3 Substrates for Methionine Production |
|
7.1.1.4 Methionine Production Strategies -- Enzymatic Conversion and Chemical Synthesis -- Fermentation -- Screening for Strains and Enhancement -- 7.1.2 l-Glutamate -- 7.1.2.1 Biosynthetic Pathway of l-Glutamate -- 7.1.2.2 Glutamate-Producing Microorganisms -- 7.1.2.3 Substrate for Glutamate Production -- 7.1.2.4 Glutamate Production Strategies -- Fermentation -- Gene Modifications -- Metabolic Flux Perusal of Glutamate Overproduction -- 7.1.3 l-Lysine -- 7.1.3.1 Biosynthetic Pathways of l-Lysine -- 7.1.3.2 l-Lysine-Producing Microorganism -- 7.1.3.3 Substrate for Lysine Production -- 7.1.3.4 Lysine Production Strategies -- Fermentation -- Genetic Engineering -- 7.1.4 Riboflavin (Vitamin B2) -- 7.1.4.1 Biosynthetic Pathway of RF -- 7.1.4.2 RF-Producing Microorganism -- 7.1.4.3 Substrate for RF Production -- 7.1.4.4 Production Strategies for RF -- Chemical Synthesis -- Biotechnological Production -- Genetic Modifications -- 7.1.5 Vitamin B12 -- 7.1.5.1 Biosynthetic Pathway for Vitamin B12 Production -- 7.1.5.2 Microorganisms Producing Vitamin B12 -- 7.1.5.3 Substrate for Producing Vitamin B12 -- 7.1.5.4 Production Strategies for Vitamin B12 -- Microbial Production of Vitamin B12 -- E. coli Cell Enzyme Transformation -- 7.1.6 Coenzyme Q10 -- 7.1.6.1 Biosynthetic Pathway of Coenzyme Q10 -- 7.1.6.2 Coenzyme Q10-Producing Microorganisms -- 7.1.6.3 Substrates for Coenzyme Q10 Production -- 7.1.6.4 Production Strategies for Coenzyme Q10 -- Chemical Synthesis Methods -- Biotechnological Production Methods for Coenzyme Q10 -- Genetic Modification -- 7.1.7 HA -- 7.1.7.1 Biosynthetic Pathway of HA -- 7.1.7.2 Microorganisms Producing HA -- 7.1.7.3 Substrates for Production of HA -- 7.1.7.4 Production Strategies for HA -- Extraction -- Fermentation -- Genetic Modification -- 7.1.8 Lactic Acid -- 7.1.8.1 Biosynthetic Pathway of LA -- 7.1.8.2 Microorganisms for the Production of LA |
| Summary |
The second volume of the Book-Industrial Microbiology and Biotechnology covers various emerging concepts in microbial technology which have been developed to harness the potential of the microbes. The book examines the microbes-based products that have widespread applications in various domains i.e., agriculture, biorefinery, bioremediation, pharmaceutical, and medical sectors. It focusses on recent advances and emerging topics such as CRISPR technology, advanced topics of genomics, including functional genomics, metagenomics, metabolomics, and structural and system biology approaches for enhanced production of industrially relevant products. It further gives an insight into the advancement of genetic engineering with special emphasis on value-added products via microalgal systems and their techno-economics analysis and life cycle assessment. The book towards the end presents recent advancements in the use of microbes for the production of industrial relevant enzymes, amino acids, vitamins, and nutraceuticals, on vaccine development and their biomedical applications. The book is an essential source for researchers working in allied fields of microbiology, biotechnology, and bioengineering |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Industrial microbiology.
|
|
Biotechnology.
|
|
bioengineering.
|
|
Biotechnology
|
|
Industrial microbiology
|
| Form |
Electronic book
|
| Author |
Verma, Pradeep, editor
|
| ISBN |
9789819928163 |
|
9819928168 |
|
