Description |
xviii, 988 pages : illustrations ; 26 cm |
Contents |
Machine derived contents note: Contents -- Preface vii -- Contributors xiii -- 1 Extraction and Solubilization of Proteins for Proteomic Studies -- Richard M. Leimgruber xx -- 2 Preparation of Bacterial Samples for 2-D PAGE -- Brian Berg Vandahl, Gunna Christiansen, and Svend Birkelund xx -- 3 Preparation of Yeast Samples for 2-D PAGE -- Joakim Norbeck xx -- 4 Preparation of Mammalian Tissue Samples for 2-D Electrophoresis -- Frank Witzmann xx -- 5 Differential Detergent Fractionation of Eukaryotic Cells -- Gregory S. Makowski xx -- 6 Serum or Plasma Sample Preparation for 2-D Gel Electrophoresis -- Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, -- and Craig D. Shriver xx -- 7 Preparation of Plant Protein Samples for 2-D PAGE -- David W. M. Leung xx -- 8 Laser-Assisted Microdissection for Sample Preparation -- Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony Sullivan, Henry Brzeski, -- Richard I. Somiari, and Craig D. Shriver xx -- 9 Purification of Cellular and Organelle Populations -- by Fluorescence-Activated Cell Sorting for Proteome Analysis -- William L. Godfrey, Colette J. Rudd, Sujata Iyer, -- and Diether Recktenwald xx -- 10 Purification of Nucleoli From Lymphoma Cells and Solubilization -- of Nucleolar Proteins for 2-DE Separations -- Regis Dieckmann, Yohanne CoutÄ, Denis Hochstrasser, -- Jean-Jacques Diaz, and Jean-Charles Sanchez xx -- 11 Prefractionation of Complex Protein Mixtures for 2-D PAGE -- Using Reversed-Phase Liquid Chromatography -- Volker Badock and Albrecht Otto xx -- 12 Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using -- ZOOM IEF Fractionators to Improve Protein Profiling -- Xun Zuo, Ki-Boom Lee, and David W. Speicher xx -- 13 Large-Format 2-D Polyacrylamide Gel Electrophoresis -- Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari, -- and Craig Shiver xx -- 14 Analysis of Membrane Proteins by 2-D Gels -- Michael Fountoulakis xx -- 15 2-D PAGE of High-Molecular-Mass Proteins -- Masamichi Oh-Ishi and Tadakazu Maeda xx -- 16 Using Ultra-Zoom Gels for High-Resolution 2-D Polyacrylamide -- Gel Electrophoresis -- Sjouke Hoving, Hans Voshol, and Jan van Oostrum xx -- 17 NEPHGE and pI Strip Proteomic 2-D Gel Electrophoretic Mapping -- of Lipid-Rich Membranes -- Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, -- and Christopher M. Taylor xx -- 18 Silver Staining of 2-D Gels -- Julia Poland, Thierry Rabilloud, and Pranav Sinha xx -- 19 Zn2+ Reverse Staining Technique -- Carlos Fernandez-Patron xx -- 20 Multiplexed Proteomics Technology for the Fluorescence Detection -- of Glycoprotein Levels and Protein Expression Levels Using Pro-Q -- Emerald and SYPRO Ruby Dyes -- Birte Schulenberg and Wayne F. Patton xx -- 21 Multiplexed Proteomics Technology for the Fluorescence Detection -- of Phosphorylation and Protein Expression Levels Using Pro-Q -- Diamond and SYPRO Ruby Dyes -- Birte Schulenberg, Terrie Goodman, Thomas Steinberg, -- and Wayne F. Patton xx -- 22 Sensitive Quantitative Fluorescence Detection of Proteins in Gels -- Using SYPRO Ruby Protein Gel Stain -- Birte Schulenberg, Nancy Ahnert, and Wayne F. Patton xx -- 23 Rapid, Sensitive Detection of Proteins in Minigels With Fluorescent Dyes: Coomassie Fluor -- Orange, SYPRO Orange, SYPRO Red, -- and SYPRO Tangerine Protein Gel Stains -- Thomas H. Steinberg, Courtenay R. Hart, and Wayne F. Patton xx -- 24 Difference Gel Electrophoresis in a High-Throughput Environment -- Stephen Russell, Stella B. Somiari, Anthony G. Sullivan, -- Darrell L. Ellsworth, Henry Brzeski, and Craig D. Shriver xx -- 25 Statistical Analysis of 2-D Gel Patterns -- Francoise Seillier-Moiseiwitsch xx -- 26 2-DE Databases on the World Wide Web -- Christine Hoogland, Khaled Mostaguir, and Ron D. Appel xx -- 27 Computer Analysis of 2-D Images -- Patricia M. Palagi, Daniel Walther, GÄrard Bouchet, Sonja Voordijk, -- and Ron D. Appel xx -- 28 Comparing 2-D Electrophoretic Gels Across Internet Databases: -- An Open Source Application -- Peter F. Lemkin, Gregory C. Thornwall, and Jai Evans xx -- 29 Sample Cleanup by Solid-Phase Extraction/Pipet Tip Chromatography -- Alastair Aitken xx -- 30 Protein Identification by In-Gel Digestion and Mass Spectrometric Analysis -- Michele Learmonth and Alastair Aitken xx -- 31 Peptide Sequences of 2-D Gel Separated Protein Spots -- by Nanoelectrospray Tandem Mass Spectrometry -- Alastair Aitken xx -- 32 Identification of Proteins by MALDI-tof MS -- Alastair Aitken xx -- 33 Sequencing of Tryptic Peptides Using Chemically-Assisted Fragmentation -- Maldi-Psd -- John Flensburg and Maria Liminga xx -- 34 The In Situ Characterization of Membrane-Immobilized 2-D PAGE -- Separated Proteins Using Ink-Jet Technology -- Patrick W. Cooley, Janice L. Joss, Femia G. Hopwood, Nicole L. Wilson, -- and Andrew A. Gooley xx -- 35 Protein Identification by Peptide Mass Fingerprinting -- Alastair Aitken xx -- 36 Analysis of the Proteomes in Human Tissues by In-Gel Isoelectric Focusing -- and Mass Spectrometry -- Francesco Giorgianni and Sarka Beranova-Giorgianni xx -- 37 Liquid Chromatograph Coupled to MS for Proteome Analysis -- Alastair Aitken xx -- 38 Quantitative Analysis of Proteomes and Subproteomes by Isotope-Coded -- Affinity Tags (ICAT) and Solid-Phase Glycoprotein Capture -- Eugene Yi, Hui Zhang, Kelly Cooke, Ruedi Aebersold, -- and David R. Goodlett xx -- 39 Amino Acid-Coded Mass Tagging for Quantitative Profiling -- of Differentially Expressed Proteins -- Xian Chen xx -- 40 Mass-Coded Abundance Tagging (MCAT) for Protein Identification -- and Relative Abundance Determination -- Gerard Cagney and Andrew Emili xx -- 41 Virtual 2-D Gel Electrophoresis by Mass Spectrometry -- Angela Walker, Gary Rymar, and Philip Andrews xx -- 42 Identification of Posttranslational Modification by Mass Spectrometry -- Alastair Aitken xx -- 43 Approaches to the O-Glycoproteome -- Franz-Georg Hanisch and Stefan Mller xx -- 44 Identification of Protein Phosphorylation Sites by Mass Spectrometry -- Alastair Aitken vv -- 45 Quantitative Analysis of Protein Phosphorylation Status and Protein Kinase Activity on -- Microassays Using Pro-Q Diamond Dye Technology -- Karen Martin and Wayne F. Patton xx -- 46 New Challenges and Strategies for Multiple Sequence Alignment -- in the Proteomics Era -- Julie D. Thompson and Olivier Poch xx -- 47 The Clustal Series of Programs for Multiple Sequence Alignment -- Julie D. Thompson xx -- 48 FASTA Servers for Sequence Similarity Search -- Biju Issac and Gajendra P. S. Raghava xx -- 49 Protein Sequence Analysis and Domain Identification -- Chris P. Ponting and Ewan Birney xx -- 50 Mammalian Genes and Evolutionary Genomics -- Leo Goodstadt and Chris Ponting xx -- 51 Computational Identification of Related Proteins: -- BLAST and PSI-BLAST and Other Tools -- Qunfeng Dong and Volker Brendel xx -- 52 Protein Identification and Analysis Tools on the ExPASy Server -- Elizabeth Gasteiger, Christine Hoogland, Alexandre Gattiker, -- SÄverine Duvaud, Mark Wilkins, Ron D. Appel xx -- 53 Protein Sequence Databases -- Michele Magrane, Maria Jesus Martin, Claire OÔDonovan, -- and Rolf Apweiler xx -- 54 In Silico Characterization of Proteins: InterPro and Proteome Analysis -- Nicola Jane Mulder, Manuela Pruess, and Rolf Apweiler xx -- 55 Computational Prediction of Protein/Protein Interactions -- Anton J. Enright, Lucy Skrabanek, and Gary D. Bader xx -- 56 The Yeast Two-Hybrid System for Detecting Interacting Proteins -- Ilya G. Serebriiskii, Erica A. Golemis, and Peter Uetz xx -- 57 Antibody-Affinity Purification to Detect Interacting Proteins -- Sonia Navarro and Lucio Comai xx -- 58 Biomolecular Interaction Analysis Coupled With Mass Spectrometry -- to Detect Interacting Proteins -- Setsuko Hashimoto, Toshiaki Isobe, and Tohru Natsume xx -- 59 Assessment of Antibody-Antigen Interaction Using SELDI Technology -- Li-Shen Hsieh, Ramy Moharram, Emilio Caputo, and Brian M. Martin xx -- 60 Protein and Peptide Microarray-Based Assay Technology -- Scott T. Clarke xx -- 61 Production of Protein Microarrays Using Robotic Pin Printing Technologies -- Ye Fang, Ann M. Ferrie, and Fang Lai xx -- 62 PCR-Directed Protein In Situ Arrays -- Joe Boutell and Minyue He xx -- 63 Site-Specific Immobilization of Proteins in a Microarray -- Yee Peng R. Lue, Su Yin D. Yeo, Lay Pheng Tan, Grace Y. J. Chen, -- and Shao Q. Yao xx -- 64 A Guide to Protein Interaction Databases -- Tiffany B. Fischer, Melissa Paczkowski, Michael F. Zettel, and Jerry Tsai xx -- 65 Deriving Function from Structure: Approaches and Limitations -- Annabel E. Todd xx -- 66 Comparative Protein Structure Modeling -- M. S. Madhusudhan, Marc A. Marti Renom, Narayanan Eswar, Bino John, -- Ursula Pieper, Rachel Karchin, Min-Yi Shen, and Andrej Sali xx -- 67 Classification of Protein Sequences and Structures -- Shalom Rackovsky xx -- 68 How to Use Protein 1D Structure Predicted by PROFphd -- Burkhard Rost xx -- 69 Classification of Proteins Folds -- Rob Russell xx -- 70 Protein Threading -- Andrew E. Torda xx -- 71 High-Throughput Crystallography for Structural Proteomics -- Jeff Yon, Mladen Vinkovic, and Harren Jhoti xx -- 72 Automated High-Throughput Protein Crystallization -- Arezou Azarani xx -- 73 NMR-Based Structure Determination of Proteins in Solution -- Andrzej Ejchart and Igor Zhukov xx -- Index xxx |
Summary |
.....Proteomics handbook offers molecular biologists, protein chemists, clincial and medicinal chemists, biochemists and microbiologists a comprehensive collection of today's best tools for understanding not only the function of individual proteins in the cell, but also for identifying new therapeutic targets |
Bibliography |
Includes bibliographical references and index |
Notes |
Also available via the World Wide Web |
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Print version record |
In |
OhioLINK electronic book center |
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SpringerLink |
Subject |
Proteomics -- Laboratory manuals.
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Proteomics -- Methods.
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Proteomics -- Handbooks, manuals, etc.
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Proteomics -- methods.
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Proteomics -- methods.
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Author |
Walker, John M., 1948-
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LC no. |
2004016126 |
ISBN |
1588293432 hardcover alkaline paper |
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1588295931 paperback alkaline paper |
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