| Description |
1 online resource (419 p.) |
| Contents |
Cover -- Title Page -- Copyright -- Contents -- Foreword by Ernst H. K. Stelzer -- Preface -- Chapter 1 Let There be Light Sheet -- 1.1 Historical Context of Light Sheet Microscopy - Ultramicroscopy -- 1.2 Light Sheet Imaging Across the Twentieth Century -- 1.3 And here Comes the Flood -- 1.4 The Building of a Community -- References -- Chapter 2 Illumination in Light Sheet Fluorescence Microscopy -- 2.1 Introduction -- 2.2 Axial Resolution and Optical Sectioning in Light Sheet Microscopy -- 2.2.1 The Point Spread Function in Fluorescence Microscopy |
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2.2.2 The Point Spread Function in Light Sheet Fluorescence Microscopy -- 2.3 Light Sheet Dimensions -- 2.3.1 Gaussian Optics Description of Beam Focusing (x,z Axes) -- 2.3.2 Methods of Light Sheet Production (y Axis) -- 2.4 Practical Light Sheet Generation -- 2.4.1 Static and Pivoted Light Sheets -- 2.4.2 Scanned Light Sheets -- 2.5 Degradation of the Light Sheet in Tissue -- 2.5.1 Absorption of the Light Sheet in Tissue -- 2.5.2 Refraction of the Light Sheet in Tissue -- 2.5.3 Scattering of the Light Sheet in Tissue -- 2.6 Challenges and Benefits of Light Sheet Modes |
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2.6.1 Parallelization of the Light Sheet -- 2.6.2 Image Artifacts Arising from Light Sheet Illumination -- 2.6.3 Homogeneity of Light Sheet Illumination -- 2.6.4 Robustness and Simplicity of Light Sheet Generation -- 2.6.5 The Merits of Static, Pivoted, and Scanned Light Sheets -- 2.7 Multiphoton Excitation -- 2.7.1 Two-Photon Light Sheets -- 2.7.2 Two-Photon Light Sheet Dimensions -- 2.7.3 Comparison with One-Photon Light Sheet Microscopy -- 2.7.4 Comparison with Laser-Scanning Multiphoton Microscopy -- 2.8 Multi-View Illumination -- 2.9 High-Resolution Imaging |
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2.9.1 Geometric Limitations for High-Resolution Imaging -- 2.9.1.1 Oblique Light Sheets -- 2.9.1.2 Reflected Light Sheets -- 2.9.2 Diffractive Limitations for High-Resolution Imaging -- 2.9.2.1 Bessel Beams -- 2.9.2.2 Axially Swept Beams -- 2.9.2.3 Photophysical Approaches -- 2.10 Conclusions -- References -- Chapter 3 A Small Guide on How to Mount a Sample in a Light-Sheet Microscope -- 3.1 Introduction -- 3.2 A Few Basic Rules -- 3.2.1 Rule 0 - Don't Panic! Become Enthusiastic! -- 3.2.2 Rule 1 - Keep it Clean -- 3.2.3 Rule 2 - The Light Comes Sideways |
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3.2.4 Rule 3 - The Theory does not Apply to your Sample -- 3.2.5 Rule 4 - Sample Geometry Matters -- 3.2.6 Rule 5 - Know Your System Well -- 3.2.7 Rule 6 - How Does Your Sample Move? -- 3.2.8 Rule 7 - What Was Lost? -- 3.2.9 Rule 8 - Consistency is Key -- 3.3 The Light-Matter Conundrum -- 3.4 Hydrogels -- 3.4.1 Preparation -- 3.5 Glues -- 3.6 Sample Holders -- 3.7 Clearing -- 3.8 Cleaning, Labelling, and Storing Samples -- 3.9 An Example: Time-lapse Live Imaging of Three-dimensional Cultures -- 3.9.1 Environmental Control: Temperature, pH, Oxygenation |
| Notes |
Description based upon print version of record |
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3.9.2 Perfusion-based Environmental Control |
| Form |
Electronic book
|
| Author |
Tomancak, Pavel
|
| ISBN |
9783527803934 |
|
3527803939 |
|
3527803904 |
|
9783527803903 |
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3527712771 |
|
9783527712779 |
|
3527803912 |
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9783527803910 |
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