Title Atomic force microscopy in biomedical research : methods and protocols / edited by Pier Carlo Braga, Davide Ricci

Published New York : Humana : Springer, ©2011

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Description 1 online resource (xiv, 508 pages) : illustrations Series Methods in molecular biology, 1940-6029 ; v. 736 Methods in molecular biology (Clifton, N.J.) ; v. 736. 1064-3745 Contents How the atomic force microscope works / Bruno Torre, Davide Ricci, and Pier Carlo Braga -- Measurement methods in atomic force microscopy / Bruno Torre [and others] -- Recognizing and avoiding artifacts in atomic force microscopy imaging / Claudio Canale [and others] -- Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy / Stewart M. Carnally, J. Michael Edwardson, and Nelson P. Barrera -- High resolution imaging of immunoglobulin G antibodies and other biomolecules using amplitude modulation atomic force microscopy in air / Sergio Santos and Neil H. Thomson -- Atomic force microscopy of ex vivo amyloid fibrils / Claudio Canale, Annalisa Relini, and Alessandra Gliozzi -- Studying collagen self-assembly by time-lapse high-resolution atomic force microscopy / Clemens M. Franz and Daniel J. Muller -- Atomic force microscopy imaging of human metaphase chromosomes in liquid / Osamu Hoshi and Tatsuo Ushiki -- Atomic force microscopy of proteasome assemblies / Maria Gaczynska and Pawel A. Osmulski -- Atomic force microscopy of isolated mitochondria / Bradley E. Layton and M. Brent Boyd -- Imaging and interrogating native membrane proteins using the atomic force microscope / Andreas Engel -- Atomic force microscopy investigation of viruses / Alexander McPherson and Yurii G. Kuznetsov -- Determination of the kinetic on- and off-rate of single virus-cell interactions / Christian Rankl [and others] -- Atomic force microscopy as a tool for the study of the ultrastructure of trypanosomatid parasites / Wanderley de Souza [and others] -- Normal and pathological erythrocytes studied by atomic force microscopy / Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer -- Growth cones of living neurons probed by the atomic force microscope / Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco -- Highlights on ultrastructural pathology of human sperm / Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna -- High-speed atomic force microscopy and biomolecular processes / Takayuki Uchihashi and Toshio Ando -- Atomic force microscopy in mechanobiology : measuring microelastic heterogeneity of living cells / Evren U. Azeloglu and Kevin D. Costa -- Force-clamp measurements of receptor-ligand interactions / Felix Rico, Calvin Chu, and Vincent T. Moy -- Measuring cell adhesion forces : theory and principles / Martin Benoit and Christine Selhuber-Unkel -- Nanoscale investigation on E. coli adhesion to modified silicone surfaces / Ting Cao [and others] -- Imaging bacterial shape, surface, and appendages before and after treatment with antibiotics / Pier Carlo Braga and Davide Ricci -- Thymol-induced alterations in Candida albicans imaged by atomic force microscopy / Pier Carlo Braga and Davide Ricci -- Atomic force microscope-enabled studies of integrin-extracellular matrix interactions in vascular smooth muscle and endothelial cells / Zhe Sun and Gerald A. Meininger -- Atomic force microscopy studies on circular DNA structural changes by vincristine and aspirin / Zhongdang Xiao [and others] -- Combined atomic force microscopy and fluorescence microscopy / Miklos S.Z. Kellermayer -- Chemical modifications of atomic force microscopy tips / Regis Barattin and Normand Voyer -- Atomic force microscopy as nanorobot / Ning Xi [and others] Summary With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies Analysis geneeskunde medicine volksgezondheid public health afbeelden imagery microscopie microscopy biologische technieken biological techniques radiologie radiology Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Atomic force microscopy. Biology -- Research. Medicine -- Research. Biochemistry. Scanning probe microscopy. Life sciences. Biology. Chemistry. Physical sciences. Diagnostic imaging. Diagnosis. Methodology. Investigative Techniques Biochemistry Microscopy, Scanning Probe Genetics Publication Formats Analytical, Diagnostic and Therapeutic Techniques and Equipment Biological Science Disciplines Biology Chemistry Publication Characteristics Microscopy Natural Science Disciplines Diagnostic Imaging Disciplines and Occupations Diagnostic Techniques and Procedures Diagnosis Laboratory Manuals Molecular Biology Methods Microscopy, Atomic Force biochemistry. biological sciences. biology. chemistry. physical sciences. diagnosis. methodology. Scanning probe microscopy Physical sciences Methodology Life sciences Diagnostic imaging Diagnosis Chemistry Biology Biochemistry Atomic force microscopy Biology -- Research Medicine -- Research Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Braga, Pier Carlo. Ricci, Davide. ISBN 9781617791055 1617791059 1617791040 9781617791048

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