Title Vascular proteomics : methods and protocols / edited by Fernando Vivanco

Published New York : Humana Press, ©2013

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xi, 230 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 1000 Methods in molecular biology (Clifton, N.J.) ; v. 1000. 1064-3745 Contents Vascular Proteomics -- Laser Microdissection and Saturation Labeling Dige Method for the Analysis of Human Arteries -- Use of TOF-SIMS in Vascular Biology -- Proteomic Analysis of Vascular Smooth Muscle Cells with S- and R-Enantiomers of Atenolol by ITRAQ and LC-MS/MS -- Phosphoproteomic Analysis of Aortic Endothelial Cells Activated by Oxidized Phospholipids -- Characterization of Membrane and Cytosolic Proteins of Erythrocytes -- Characterization and Analysis of Human Arterial Tissue Secretome by 2-DE and NLC-MS/MS -- Identification of Novel Biomarkers of Abdominal Aortic Aneurysms (AAA) by 2D-DIGE and MALDI-MS FROM AAA-Thrombus Conditioned Media -- Metabolites Secreted by Human Atherothrombotic Aneurysm -- Quantitative Analysis of Apolipoproteins in Human HDL by Top-Down Differential Mass Spectrometry -- Quantitative Proteomics Analysis of High-Density Lipoproteins by Stable 18O-Isotope Labeling -- Unraveling Biomarkers of Abdominal Aortic Aneurisms by iTRAQ Analysis of Depleted Plasma -- Absolute Quantitation of Proteins in Human Blood by Multiplexed Multiple Reaction Monitoring Mass Spectrometry -- Multiple Reaction Monitoring (MRM) of Plasma Proteins in Cardiovascular Proteomics -- Proteomic Analysis of Plasma of Patients with Left Ventricular Remodeling After Myocardial Infarction. Usefulness of SELDI-TOF -- Proteomic Analysis of Urinary Exosomes in Cardiovascular and Associated Kidney Diseases by Two Dimensional Electrophoresis and LC-MS/MS Summary Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research Analysis Life sciences Cardiology Biochemistry Protein Science eiwitexpressieanalyse proteomics eiwitten proteins cardiologie Proteomics Bibliography Includes bibliographical references and index Notes English Subject Cardiovascular system -- Molecular aspects -- Laboratory manuals Proteomics -- Laboratory manuals Diseases. Genomics. Biochemistry. Biological products. Biologicals. Cardiovascular system. Metabolism. Chemistry. Anatomy. Bioinformatics. Computational biology. Life sciences. Biology. Physical sciences. Biochemical markers. Proteomics. Blood-vessels. Methodology. Cardiovascular system -- Diseases. Disease Genomics Biochemistry Biological Factors Cardiovascular System Publication Formats Investigative Techniques Metabolism Chemistry Phenomena and Processes Anatomy Computational Biology Chemicals and Drugs Biological Science Disciplines Publication Characteristics Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetics Biology Natural Science Disciplines Disciplines and Occupations Biomarkers Proteomics Laboratory Manuals Blood Vessels Methods Cardiovascular Diseases Animal Structures Biological Products illness. disease. biochemistry. metabolism (biological concept) chemistry. anatomy. biological sciences. biology. physical sciences. methodology. Physical sciences Methodology Metabolism Life sciences Genomics Diseases Computational biology Chemistry Cardiovascular system -- Diseases Cardiovascular system Blood-vessels Biology Biologicals Biological products Bioinformatics Biochemistry Biochemical markers Anatomy Proteomics Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Vivanco, Fernando, 1948- ISBN 9781627034050 1627034056 1627034048 9781627034043

  Permalink