Description |
online resource (streaming video file)(322 seconds) |
Series |
Bioengineering |
Summary |
Here, we present a protocol to measure the virulence of planktonic or surface-attached bacteria using D. discoideum (amoeba) as a host. Virulence is measured over a period of 1 h and host killing is quantified using fluorescence microscopy and image analysis. We demonstrate this protocol using the bacterium P. aeruginosa |
Notes |
Title from resource description page |
Audience |
For undergraduate, graduate, and professional students |
Notes |
English |
Subject |
Bioengineering
|
Form |
Streaming video
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