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Title RNA turnover in prokaryotes, archae, and organelles / edited by Lynne E. Maquat, Cecilia Arraiano
Published Amsterdam ; Boston : Elsevier/Academic Press, [2008]
©2008
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Description 1 online resource (1 pages, 564 pages) : illustrations
Series Methods in enzymology, 0076-6879 ; v. 447
Methods in enzymology ; v. 447
Contents Analysis of RNA decay, processing, and polyadenylation in Escherichia coli and other prokaryotes / Bijoy K. Mohanty ... [et al.] -- Analyzing the decay of stable RNAs in E. coli / Zhongwei Li and Murray P. Deutscher -- Genomic analysis of mRNA decay in E. coli with DNA microarrays / Pei-Hsun Lin ... [et al.] -- Co-immunopurification of multiprotein complexes containing RNA-degrading enzymes / Agamemnon J. Carpousis ... [et al.] -- PABLO analysis of RNA : 5'-phosphorylation state and 5'-end mapping / Helena Celesnik, Atilio Deana, and Joel G. Belasco -- A proteomic approach to the analysis of RNA degradosome composition in Escherichia coli / Pierluigi Mauri and Gianni Dehò -- New approaches to understanding double-stranded RNA processing by Ribonuclease III : purification and assays of homodimeric and heterodimeric forms of RNase III from bacterial extremophiles and mesophiles / Wenzhao Meng ... [et al.] -- Characterizing ribonucleases in vitro : examples of synergies between biochemical and structural analysis / Cecília Maria Arraiano, Ana Barbas, and Mónica Amblar -- The role of RNA chaperone Hfq in poly(A) metabolism : methods to determine positions, abundance, and lengths of short oligo(A) tails / Philippe Régnier and Eliane Hajnsdorf -- Assaying DEAD-box RNA helicases and their role in mRNA degradation in Escherichia coli / Agamemnon J. Carpousis, Vanessa Khemici, and Leonora Poljak -- Preparation of the Escherichia coli RNase E protein and reconstitution of the RNA degradosome / George A. Mackie ... [et al.] -- Identifying and characterizing substrates of the RNase E/G family of enzymes / Louise Kime, Stefanie S. Jourdan, and Kenneth J. McDowall -- Construction and characterization of E. coli K12 strains in which the transcription of selected genes is desynchronized from translation / Florence Proux and Marc Dreyfus -- Analysis of mRNA decay in Bacillus subtilis / David H. Bechhofer ... [et al.] -- Assay of Bacillus subtilis ribonucleases in vitro / Ciarán Condon ... [et al.] -- Staphylococcus aureus Endoribonuclease III : purification and properties / Clément Chevalier ... [et al.] -- Studying tmRNA-mediated surveillance and nonstop mRNA decay / Thomas Sundermeier ... [et al.] -- Analyses of mRNA destabilization and translational inhibition mediated by Hfq-binding small RNAs / Teppei Morita ... [et al.] -- In vivo and in vitro studies of RNA degrading activities in Archaea / Elena Evguenieva-Hackenberg, Steffen Wagner, and Gabriele Klug -- Expression, reconstitution, and structure of an archaeal RNA degrading exosome / Esben Lorentzen and Elena Conti -- Polyadenylation-mediated RNA degradation in plant mitochondria / Sarah Holec ... [et al.] -- In vivo and in vitro approaches for studying the yeast mitochondrial RNA degradosome complex / Michal Malecki ... [et al.] -- Measuring mRNA decay in human mitochondria / Asuteka Nagao, Narumi Hino-Shigi, and Tsutomu Suzuki -- Detection and characterization of polyadenylated RNA in eukarya, bacteria, archaea, and organelles / Shimyn Slomovic, Victoria Portnoy, and Gadi Schuster -- RNA decay by messenger RNA interferases / Mikkel Christensen-Dalsgaard ... [et al.]
Bibliography Includes bibliographical references and indexes
Notes Also available via the World Wide Web
System requirements: Internet connectivity, World Wide Web browser, and Adobe Acrobat reader
Subject Cell organelles.
Genetic regulation.
Messenger RNA.
Prokaryotes.
RNA-protein interactions.
Form Electronic book
Author Arraiano, Cecilia M. (Cecilia Maria), 1959-
Maquat, Lynne.
ISBN 0080922066 (electronic bk.)
012374377X
9780080922065 (electronic bk.)
9780123743770