| Description |
online resource (streaming video file)(800 seconds) |
| Series |
Immunology and Infection |
| Summary |
We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental procedures where a specific DNAzyme probe denoted "RFD-EC1" is employed for the detection of the model bacterium, Escherichia coli (E. coli) |
| Notes |
Title from resource description page |
| Audience |
For undergraduate, graduate, and professional students |
| Notes |
English |
| Subject |
Immunology and Infection
|
| Form |
Streaming video
|
|
