Title Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published Cambridge, MA MyJoVE Corp 2016

Online access available from: Journal of Visualized Experiments    View Resource Record

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Description 1 online resource (streaming video file) (439 secs.) Series Biology Summary MicroRNAs (miRNAs) are important regulators of gene expression and have been shown to play a role in numerous biological processes. To better understand miRNA-UTR interactions, we have created a genome-wide collection of 3 UTR luciferase reporters paired with a novel luciferase gene and assay reagent, the LightSwitch system Audience For undergraduate, graduate, and professional students Notes English Description based on online resource; title from resource description page Subject Biology Form Streaming audio

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