| Description |
1 online resource (x, 158 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 411 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 411
|
| Contents |
Methodologies for staining and visualization of [beta]-galactosidase in mouse embryos and tissues / Siobhan Loughna and Deborah Henderson -- Immunohistochemical detection of [beta]-galactosidase or green fluorescent protein on tissue sections / Philip A. Seymour and Maike Sander -- Detection of reporter gene expression in murine airways / Maria Limberis, Peter Bell, and James M. Wilson -- Three-dimensional analysis of molecular signals with episcopic imaging techniques / Wolfgang J. Weninger and Timothy J. Mohun -- Fluorescent proteins for cell biology / George H. Patterson -- Detection of GFP during nervous system development in Drosophila melanogaster / Karin Edoff, James S. Dods, and Andrea H. Brand -- Autofluorescent proteins for flow cytometry / Charles G. Bailey and John E.J. Rasko -- Fluorescent protein reporter systems for single-cell measurements / Steven K. Dower, Eva E. Qwarnstrom, and Endre Kiss-Toth -- Subcellular imaging of cancer cells in live mice / Robert M. Hoffman -- Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects / Pritha Ray and Sanjiv Sam Gambhir -- Green fluorescent protein as a tracer in chimeric tissues : the power of vapor fixation / Harald Jockusch and Daniel Eberhard |
| Summary |
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists |
| Analysis |
reporter genes |
|
celbiologie |
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cellular biology |
|
merkergenen |
|
marker genes |
|
biologische technieken |
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biological techniques |
|
zoogdieren |
|
mammals |
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moleculaire merkers |
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molecular markers |
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protocollen |
|
protocols |
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Molecular Genetics |
|
Moleculaire genetica |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Reporter genes.
|
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Mammals -- Cytology.
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Mammals -- Genetics
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Cytology -- Technique.
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Cytological Techniques
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Genes, Reporter
|
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Mammals -- genetics
|
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Cytological Techniques.
|
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Mammals -- Genetics.
|
|
Genes, Reporter.
|
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Cytology -- Technique
|
|
Mammals -- Cytology
|
|
Mammals -- Genetics
|
|
Reporter genes
|
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Genanalyse
|
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Markierungsgen
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Methode
|
| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
|
|
Aufsatzsammlung.
|
| Form |
Electronic book
|
| Author |
Anson, Donald S.
|
| ISBN |
9781597455497 |
|
1597455490 |
|
1281136964 |
|
9781281136961 |
|
9786611136963 |
|
6611136967 |
|
1603270728 |
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9781603270724 |
|
