| Description |
1 online resource (streaming video file) (898 secs.) |
| Series |
Biology |
| Summary |
Affinity purification of tagged proteins in combination with mass spectrometry (APMS) is a powerful method for the systematic mapping of protein interaction networks and for investigating the mechanistic basis of biological processes. Here, we describe an optimized sequential peptide affinity (SPA) APMS procedure developed for the bacterium Escherichia coli that can be used to isolate and characterize stable multi-protein complexes to near homogeneity even starting from low copy numbers per cell |
| Audience |
For undergraduate, graduate, and professional students |
| Notes |
English |
|
Description based on online resource; title from resource description page |
| Subject |
Biology
|
| Form |
Streaming audio
|
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