| Description |
1 online resource (xiii, 328 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 84 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 84
|
| Contents |
Peptide recognition mechanisms of eukaryotic signaling modules / Chi-Hon Lee, David Cowburn, John Kuriyan -- Protein-protein interactions in signaling cascades / Bruce J. Mayer -- Transmembrane signaling by receptor oligomerization / Mark A. Lemmon, Joseph Schlessinger -- Use of peptide libraries to determine optimal substrates of tyrosine kinases / Perry M. Chan, W. Todd Miller -- Mapping the specificity of SH3 domains with phage-displayed random-peptide libraries / Andrew B. Sparks, James E. Rider, Brian K. Kay -- Selective antagonism of receptor signaling using antisense RNA to deplete G-protein subunits / Paul R. Albert, Stephen J. Morris -- Microinjection of antisense oligonucleotides and electrophysiological recording of whole-cell currents as tools to identify specific G-protein subtypes coupling hormone receptors to voltage-gated calcium channels / Vadim E. Degtiar, Burghardt Wittig, Günter Schultz, Frank Kalkbrenner -- Oocytes microinjection assay to study the MAP-kinase cascade / Juan Carlos Lacal -- Mammalian cell microinjection assay to study the function of Rac and Rho / Anne J. Ridley -- Identification and functional reconstitution of effector proteins for the GTPases Rac and CDC42Hs / Arie Abo -- Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade / Kazuya Shimizu, Toshihisa Ohtsuka, Yoshimi Takai -- Reconstitution system based on cytosol-depleted cells to study the regulation of phospholipases C and D / Shamshad Cockcroft -- Two-hybrid analysis of Ras-Raf interactions / Linda Aelst -- Cloning and mutational analysis of the Shc-phosphotyrosine interaction/ phosphotyrosine-binding domain / Vijay Yajnik, Pamela Blaikie, Ben Margolis -- Use of fluorescence spectroscopy to study the regulation of small G proteins / Tyzoon Nomanbhoy, Richard A. Cerione -- Prenylation assays for small GTPases / Miguel C. Seabra, Guy L. James -- Analysis of myristoylated and palmitoylated Src family proteins / Amy Wolven, Wouter van't Hof, Marilyn D. Resh -- Ultracentrifugation technique for measuring the binding of peptides and proteins to sucrose-loaded phospholipid vesicles / Carolyn A. Buser, Stuart McLaughlin -- Biochemical and biological analyses of farnesyl-protein transferase inhibitors / Nancy E. Kohl, Kenneth S. Koblan, Charles A. Omer, Allen Oliff, Jackson B. Gibbs -- Identification and characterization of small GTPase-associated kinases / Edward Manser, Thomas Leung, Louis Lim -- Functional studies of dual-specificity phosphatases / Hong Sun |
| Summary |
Transmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field |
| Analysis |
signaaltransductie |
|
signal transduction |
|
transmembraaneiwitten |
|
transmembrane proteins |
|
signalen |
|
signals |
|
signaalpeptide |
|
signal peptide |
|
celmembranen |
|
cell membranes |
|
biologische technieken |
|
biological techniques |
|
protocollen |
|
protocols |
|
Molecular Biology (General) |
|
Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Cellular signal transduction -- Laboratory manuals
|
|
Cellular signal transduction.
|
|
Signal Transduction
|
|
Cellular signal transduction.
|
| Genre/Form |
Laboratory manuals.
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Bar-Sagi, Dafna.
|
| ISBN |
9781592595686 |
|
1592595685 |
|
1280836865 |
|
9781280836862 |
|
