| Description |
1 online resource (xxi, 494 pages, [8] pages of color plates) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 506 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 506
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Springer protocols (Series)
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| Contents |
Immunomagnetic enrichment of human and mouse hematopoietic stem cells for gene therapy applications / Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren -- Isolation of human and mouse hematopoietic stem cells / Yuk Yin Ng [and others] -- Murine hematopoietic stem cell transduction using retroviral vectors / Ute Modlich [and others] -- Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials / Tulin Budak-Alpdogan and Isabelle Rivière -- Short-term culture of human CD34 cells for lentiviral gene transfer / Francesca Santoni de Sio and Luigi Naldini -- T cell culture for gammaretroviral transfer / Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer -- Retroviral transduction of murine primary T lymphocytes / James Lee, Michel Sadelain, and Renier Brentjens -- Lentiviral vector gene trasfer into human T cells / Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset -- DNA transposons for modification of human primary T lymphocytes / Xin Huang [and others] -- Retroviral gene transfer into primary human natural killer cells / Evran Alici, Tolga Sutlu, and M. Sirac Dilber -- Lentiviral vector-mediated genetic programming of mouse and human dendritic cells / Renata Stripecke |
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In situ (in vivo) gene transfer into murine bone marrow stem cells / Dao Pan -- In vivo and ex vivo gene transfer in thymocytes and thymocyte precursors / Oumeya Adjali [and others] -- Design and production of retro- and lentiviral vectors for gene expression in hematopoietic cells / Axel Schambach, William P. Swaney, and Johannes C.M. van der Loo -- Knock-down of gene expression in hematopoietic cells / Michaela Scherr, Letizia Venturiani, and Matthias Eder -- The use of retroviral vectors for tet-regulated gene expression in cell populations / Rainer Löw -- Detection of replication competent retrovirus and lentivirus / Lakshmi Sastry and Kenneth Cornetta -- Release testing of retroviral vectors and gene-modified cells / Diana Nordling, Anne Kaiser, and Lilith Reeves -- Copy number determination of genetically-modificed hematopoietic stem cells / Todd Schuesler [and others] -- Tissue procurement for molecular studies using laser-assisted microdissection / Ulrich Lehmann and Hans Kreipe -- Leukemia diagnosis in murine bone marrow transplantation models / Zhixiong Li, Ute Modlich, and Anjali Mishra |
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Humanized mouse models to study the human haematopoietic stem cell compartment / Dominique Bonnet -- Canine models of gene-modified hematopoiesis / Brian C. Beard and Hans-Peter Kiem -- Detection of retroviral integration sites by linear amplification-mediated PCR and tracking of individual integration clones in different samples / Manfred Schmidt [and others] -- Retroviral insertion site analysis in dominant haematopoietic clones / Olga S. Kustikova, Ute Modlich, and Boris Fehse -- Tracking gene-modified T cells in vivo / Alessandra Recchia and Fulvio Mavilio -- DNA microarray studies of hematopoietic subpopulations / Karin Pike-Overzet [and others] -- Quantification of genomic mutations in murine hematopoietic cells / Hartmut Geiger [and others] -- Proteomics studies after hematopoietic stem cell transplantation / Eva M. Weissinger, Petra Zürbig, and Arnold Ganser -- Spectral karyotyping and fluorescence in situ hybridization of murine cells / Cornelia Rudolph and Brigitte Schlegelberger -- Database setup for preclinical studies of gene-modified hematopoiesis / Brenden Balcik, Elke Grassman, and Lilith Reeves -- The US and EU regulatory perspectives on the clinical use of hematopoietic stem/progenitor cells genetically modified ex vivo by retroviral vectors / Carolyn A. Wilson and Klaus Cichutek |
| Summary |
With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells |
| Analysis |
bloedvorming |
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haematopoiesis |
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cellen |
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cells |
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dna modification |
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genetische modificatie |
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genetic engineering |
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laboratoriummethoden |
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laboratory methods |
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protocollen |
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protocols |
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Cellular Biology |
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Celbiologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Hematopoietic stem cells.
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Hematopoietic Stem Cells
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Genetic Therapy -- methods
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Hematopoietic stem cells
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Baum, Christopher, 1962-
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| ISBN |
9781597454094 |
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1597454095 |
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