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Author Biesbrock, Anna M

Title Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description online resource (streaming video file)(772 seconds)
Series Immunology and Infection
Summary Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Immunology and Infection
Form Streaming video