Title Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death

Published Cambridge, MA MyJoVE Corp 2016

Online access available from: Journal of Visualized Experiments    View Resource Record

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Description 1 online resource (streaming video file) (834 secs.) Series Biology Summary Contribution of the ACF chromatin remodeling factor to E4orf4-induced cell death was measured. The protocol includes selection of cell clones in which doxycycline treatment induces conditional knockdown of the ACF subunits Acf1 and SNF2h, and use of the DAPI assay to measure E4orf4-induced cell death in the inducible cell lines Audience For undergraduate, graduate, and professional students Notes English Description based on online resource; title from resource description page Subject Biology Form Streaming audio

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