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Author Broggi, Maria A. S

Title Isolation of Murine Lymph Node Stromal Cells
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description online resource (streaming video file)(347 seconds)
Series Immunology and Infection
Summary Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Immunology and Infection
Form Streaming video