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Title Microbiological quality assurance : a guide towards relevance and reproducibility of inocula / edited by Michael R.W. Brown, Peter Gilbert
Published Boca Raton, FL : CRC Press, 2018

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Description 1 online resource : illustrations
Series CRC revivals
CRC revivals.
Contents Cover; Half Title; Title; Copyright; Preface; The Editors; Contributors; Table Of Contents; GROWTH CONDITIONS; Chapter 1.1: Influence of the Environment on the Properties of Vegetative Microorganisms: An Overview; Introduction; Phenotypic Flexibility; Growth Rate and Nutient Deprivation; Growth as Adherent Biofilms; Solid or Liquid Culture for Conventional Susceptibility Testing; Conclusions; References; Chapter 1.2: Definition of Phenotype in Batch Culture; Introduction; Specific Nutrient Depletion in Batch Culture; Defined Nutrient-Depleted Media; An Empirical Approach
A Quantitative ApproachUSP Preservative Test Organisms; References; Chapter 1.3: Changes in Bacterial Cell Properties in Going from Exponential Growth to Stationary Phase; Introduction; Changes that Result from Starvation; Regulation of Gene Expression in Stationary Phase; Population Changes during Starvation; Acknowledgments; References; Chapter 1.4: Continuous Culture of Microorganisms in Liquid Media: Effects of Growth Rate and Nutrient Limitation; Introduction; Continuous Culture: Theoretical Considerations; Carbon Limitation; Magnesium and Iron Limitation; Oxygen Limitation; Turbidostats
Continuous Culture: Practical ConsiderationsCulture Homogeneity; Foaming; Floccules; Biofilms; Continuous Culture Fermenter Design; Modulation of Antimicrobial Susceptibility in Continuous Culture; Susceptibility Towards Chemical Biocides; Susceptibility Towards Antibiotics; References; Chapter 1.5: The Influence of Nutrient Environment During Vegetative Growth on the Properties of Bacterial Endospores; Introduction; Sporulation Medium and Definition of Spore Phenotype; Sporulation Temperature; Growth and Sporulation at Various pH; Culture Age Prior to Harvesting; References
Chapter 1.6: Influence of the Environment on the Properties of Microorganisms Grown in Association with SurfacesIntroduction; Surface Colonization and Growth; Resistance Properties of Bacterial Biofilms; Influence of Surface Attachment and Growth upon Microbial Activity; Role of the Glycocalyx; The Glycocalyx as a Moderator of Drug/Biocide Resistance; Attachment-Specific Physiologies; Quorum Sensing Transcriptional Activation; In Vitro Models of Biofilms; Closed Growth Models; Solid:Air Interfaces; Solid: Liquid Interfaces; Open Growth Models (Non-Steady-State); Chemostat-Based Models
The Robbins DeviceAnnular Reactor; Open Growth Models (Steady-State); Constant Thickness Biofilms; Perfused Biofilms; Conclusions; References; Chapter 1.7: Sources of Biological Variation and Lack of Inoculum Reproducibility: A Summary; Culture Origins; Growth Conditions of the Inoculum; Dormancy; Post-Growth Handling Procedures; Procedureffest Conditions; POST -GROWTH CONDITIONS; Chapter 2.1: The Preservation of Inocula; Introduction; Reproducibility; Biochemical Properties; Genetic Stability; Preservation by Cooling; Behavior of Solutes During Freezing; Rates of Cooling
Summary "Microbiological Quality Assurance: A Guide Towards Relevance and Reproducibility of Inocula sheds light on the difficulties of obtaining results in the test tube that will be reproducible and relevant for a wide variety of tests. This book explores the current state of research in this area and troubleshoots the problems that may be encountered in setting up appropriate cultures. The text divides naturally into three sections-growth conditions, post-growth conditions, and applications. This book serves as a valuable resource for clinical microbiologists, pharmacologists, and anyone doing in vitro experiments."--Provided by publisher
Notes Slow"" Cooling Injury
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Microbial biotechnology.
Microbial inoculants -- Quality control
SCIENCE -- Chemistry -- Industrial & Technical.
TECHNOLOGY & ENGINEERING -- Chemical & Biochemical.
Microbial biotechnology
Microbial inoculants -- Quality control
Form Electronic book
Author Brown, Michael R. W. (Michael Robert Withington), 1931- editor.
Gilbert, Peter, 1951- editor.
ISBN 9781351083003
1351083007
9781351074551
1351074555