| Description |
1 online resource (xvii, 398 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1955 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1955. 1064-3745
|
| Contents |
Preface ... Table of Contents ... Contributing Authors ... 1. Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex GenomeJoão Luís Reis-Cunha and Daniella C. Bartholomeu 2. Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine DevelopmentChristian Teh-Poot and Eric Dumonteil 3. Transcriptome Studies in Trypanosoma cruzi using RNAseqGonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, and Carlos Robello 4. An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon GeneAlicia Majeau, Claudia Herrera, and Eric Dumonteil 5. Genome Editing by CRISPR/Cas9 in Trypanosoma cruziNoelia Lander, Miguel A. Chiurillo, and Roberto Docampo 6. Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, and Sébastien Charneau 7. Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruziIzadora Volpato Rossi, Bruno Gavinho, and Marcel Ivan Ramirez 8. Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, and Mariana Potenza 9. Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante, Gaspar E. Cánepa, Carlos A. Buscaglia, and María de los Milagros Cámara 10. Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-SialidaseGiannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia, Oscar Campetella, and Juan Mucci 11. Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander Ward, Francisco Olmo, Michael D. Lewis, and John M. Kelly 12. Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host TissuesMatías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, and Valeria Tekiel 13. In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent ReportersFernando Sánchez-Valdéz and Angel Padilla 14. Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal ModelsJulien Santi-Rocca, Núria Gironès, and Manuel Fresno 15. MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected MiceLudmila Rodrigues Pinto Ferreira 16. Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR Otacilio C. Moreira and Juan Carlos Ramirez 17. Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCRJuan Carlos Ramírez and Otacilio da Cruz Moreira 18. Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase Leguizamón María Susana 19. Analytics of Functional Autoantibodies in Patients with Chagas' DiseaseGerd Wallukat, Katrin Wenzel, and Ingolf Schimke 20. Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas DiseaseElizabeth Ruiz-Lancheros, Makan Golizeh, and Momar Ndao 21. Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in PlasmaJulio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, and María-Jesús Pinazo 22. Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of #xF061;-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas DiseaseUriel Ortega-Rodriguez, Susana Portillo, Roger A. Ashmus, Jerry A. Duran, Nathaniel S. Schocker, Eva Iniguez, Alba L. Montoya, Brenda G. Zepeda, Janet J. Olivas, Nasim H. Karimi, Julio Alonso-Padilla, Luis Izquierdo, Maria-Jesús Pinazo, Belkisyolé Alarcón de Noya, Oscar Noya, Rosa A. Maldonado, Faustino Torrico, Joaquim Gascón, Katja Michael, and Igor C. Almeida 23. Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease PatientsNayara I. Medeiros and Juliana A.S. Gomes 24. Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, and Karina A. Gómez 25. Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas DiseaseMaría Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, and Susana Adriana Laucella 26. CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas DiseasePaola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, and Concepción Puerta 27. Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease John E. Wiktorowicz, M. Paola Zago, and Nisha J. Garg 28. Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected HeartsNatalia Eberhardt, Liliana María Sanmarco, and Maria Pilar Aoki |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed March 25, 2019) |
| Subject |
Trypanosoma cruzi -- Laboratory manuals
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|
Trypanosoma cruzi
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Gómez, Karina Andrea, editor
|
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Buscaglia, Carlos Andrés, editor
|
| ISBN |
9781493991488 |
|
1493991485 |
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