| Description |
1 online resource (x, 244 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 689 |
|
Springer protocols |
|
Methods in molecular biology (Clifton, N.J.) ; v. 689
|
|
Springer protocols (Series)
|
| Contents |
Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model / Hélio Chiarini-Garcia, Gleydes Gambogi Parreira, and Fernanda R.C.L. Almeida -- Histological processing of teeth and periodontal tissues for light microscopy analysis / Gerluza Aparecida Borges Silva, Adriana Moreira, and José Bento Alves -- Large plant samples: how to process for GMA embedding / Élder Antônio Sousa Paiva, Sheila Zambello de Pinho, and Denise Maria Trombert Oliveira -- Image cytometry: nuclear and chromosomal DNA quantification / Carlos Roberto Carvalho, Wellington Ronildo Clarindo, and Isabella Santiago Abreu -- Histological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection / Daniela L. Fabrino, Grazielle A. Ribeiro, Livia Teixeira, and Rossana C.N. Melo -- Intravital microscopy to study leukocyte recruitment in vivo / Vanessa Pinho [and others] -- Introduction to fluorescence microscopy / Ionita C. Ghiran -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals / Ernani Amaral, Silvia Guatimosim, and Cristina Guatimosim -- Imaging lipid bodies within leukocytes with different light microscopy techniques / Rossana C.N. Melo [and others] -- Eicosa cell: an immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer / Robert M. Hoffman -- Imaging calcium sparks in cardiac myocytes / Silvia Guatimosim, Cristina Guatimosim, and Long-Sheng Song -- Light microscopy in aquatic ecology: methods for plankton communities studies / Maria Carolina S. Soares [and others] -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedded samples / Shin-ichi Iwasaki and Hidekazu Aoyagi |
| Summary |
Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology"!series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell |
| Analysis |
microscopie |
|
microscopy |
|
biologische technieken |
|
biological techniques |
|
Biological Techniques |
|
Biologische technieken |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Microscopy.
|
|
Microscopy -- methods
|
|
Microscopy, Fluorescence -- methods
|
|
Microscopy
|
|
microscopy.
|
|
Microscopy
|
| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Chiarini-Garcia, Hélio
|
|
Melo, Rossana C
|
| ISBN |
9781607619505 |
|
1607619504 |
|
9781607619499 |
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1607619490 |
|
