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Author Cohen, Itamar

Title Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (467 secs.)
Series Biology
Summary Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Biology
Form Streaming audio