| Description |
1 online resource : illustrations |
| Contents |
Chapter 1. Introduction -- Chapter 2. Fundamental concepts -- Chapter 3. Sample preparation -- Chapter 4. Staining protocols -- Chapter 5. Microphotography and image processing: Creating artwork -- Chapter 6. Identification of major histopathological traits -- Chapter 7. Scoring and data processing -- Chapter 8. Common problems and troubleshooting -- Appendix A. Fixative solutions -- Appendix B. Dyes -- Appendix C. Accessory solutions -- Appendix D. Rapid protocols -- Appendix E. Accessory tables -- References -- Index |
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Front Cover; The Handbook of Histopathological Practices in Aquatic Environments; Copyright Page; Contents; Foreword; Preface; Acknowledgments; List of Abbreviations and Acronyms; 1 Introduction; 1.1 A Brief Account on the History and Purpose of Histology and Histopathology in Aquatic Organisms; 1.2 Histopathological Traits as Biomarkers in Marine Environmental Science; 1.3 Target Species and Organ: The Importance of Understanding Toxicological Pathways; 1.4 Histopathology Links Many Levels of Biological Organization: The Systems Biology Approach; 1.5 How to Use This Book |
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2 Fundamental Concepts2.1 Procedural Overview; 2.2 Basic Equipment and Material; 2.2.1 Essential Equipment; 2.2.2 Glass and Plasticware for Sample Preparation; 2.2.3 Microscope slides, Coverslips, and Adhesives; 2.2.4 Microtome Knives and Blades; 2.3 Important Considerations on Solvents and Reagents; 2.3.1 Fixatives; 2.3.2 Embedding Media; 2.3.3 Histological Dyes; 2.3.4 Waters; 2.3.5 Alcohols and Other Solvents; 2.3.6 Buffer Systems; 2.3.7 Mounting Media; 2.4 Safety Precautions; 3 Sample Preparation; 3.1 Tissue Preparation and Fixation; 3.1.1 Harvesting Material; 3.1.2 Fixation |
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3.1.3 Additional Treatments3.2 Paraffin-Embedded Samples; 3.2.1 General Procedure for Paraffin Infiltration and Embedding; 3.2.2 Casting Paraffin Blocks; 3.2.3 Sectioning Paraffin Sections With a Rotary Microtome; 3.2.4 Staining Paraffin-Embedded Samples; 3.3 Procedures for Resin Embedding; 3.3.1 Embedding Samples in Resins; 3.3.2 Obtaining Semithin and Thin Section From Resin-Embedded Samples; 3.3.3 Staining and Contrasting Resin Sections; 4 Staining Protocols; 4.1 General Histological Stains; 4.1.1 Hematoxylin and Eosin; 4.1.1.1 Introduction; 4.1.1.2 Procedure; 4.1.2 Toluidine Blue |
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4.1.2.1 Introduction4.1.2.2 Procedure; 4.2 Histochemistry of Lipids and Sugars; 4.2.1 Alcian Blue/Nuclear Fast Red Stain for Acidic Polysaccharides; 4.2.1.1 Introduction; 4.2.1.2 Procedure; 4.2.2 Lipid Staining With Osmium Tetroxide; 4.2.2.1 Introduction; 4.2.2.2 Procedure; 4.2.3 Periodic Acid/Schiff's Stain for Polysaccharides; 4.2.3.1 Introduction; 4.2.3.2 Procedure; 4.2.4 Sudan Black for Lipids; 4.2.4.1 Introduction; 4.2.4.2 Procedure; 4.3 Peptide Histochemistry; 4.3.1 Coomassie Blue Stain for Proteins; 4.3.1.1 Introduction; 4.3.1.2 Procedure |
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4.4 Histochemistry of Metals, Metalloids, and Metallic Compounds4.4.1 Some Important Considerations on Metal Histochemistry; 4.4.2 Castel's Method for Arsenic; 4.4.2.1 Introduction; 4.4.2.2 Procedure; 4.4.3 Perl's Prussian Blue for Hemosiderin; 4.4.3.1 Introduction; 4.4.3.2 Procedure; 4.4.4 Rubeanic Acid Method for Copper; 4.4.4.1 Introduction; 4.4.4.2 Procedure; 4.4.5 von Kossa's Stain for Calcium; 4.4.5.1 Introduction; 4.4.5.2 Procedure; 4.5 Stains for Microorganisms; 4.5.1 Gram's Stain for Paraffin Sections; 4.5.1.1 Introduction; 4.5.1.2 Procedure; 4.5.2 Staining Acid-Fast Microorganisms |
| Summary |
"[This work] offers readers in aquatic biology and other water-based environmental sciences a comprehensive resource on histopathology, which is a key tool in the growing field of ecotoxicology. This work brings together the necessary knowledge, from sample preparation, to trait identification, and scoring and data treatment. Furthermore, with examples from several groups of organisms (from worms to fish), these practices can be applied across a wide array of aquatic ecosystems. This book provides a step-by-step approach to solving the questions researchers encounter in aquatic biology and related fields."--Https://www.elsevier.com |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Water -- Pollution -- Toxicology.
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Histology, Pathological.
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Histology.
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Environmental toxicology.
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Ecotoxicology
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Water Pollution -- analysis
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Aquatic Organisms
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Histology
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Pathology
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SCIENCE -- Life Sciences -- Anatomy & Physiology.
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Histology
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Histology, Pathological
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Water -- Pollution -- Toxicology
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| Form |
Electronic book
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| ISBN |
9780128120316 |
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0128120312 |
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