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Author Daniele, Stefano G

Title Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (552 secs.)
Series Immunology and Infection
Summary One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam)
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Immunology and Infection
Form Streaming audio