| Description |
1 online resource (124 p.) |
| Contents |
2. Before you load the DNA on to the gel -- 3. Preparing and running the gel -- 3.1 Agarose -- 3.2 Electrophoresis buffers -- 3.3 Casting the gel -- 3.4 Assembling the gel electrophoresis tank -- 3.5 Loading your samples -- 3.6 Running the gel -- 3.7 Visualizing the DNA with UV radiation -- 3.8 Photographing your gel -- 3.9 Interpretation of gels -- 3.9.1 Genomic DNA -- 3.9.2 Cloned DNA -- 3.10 What can go wrong with electrophoresis? -- 4. Further reading -- 5. References -- Chapter 3 Southern blotting II: performing the blot |
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1. Uni-directional capillary blotting on to a single membrane at neutral pH -- 1.1 Preparing the gel for blotting -- 1.1.1 Partial depurination -- 1.1.2 Denaturation -- 1.1.3 Neutralization -- 1.2 Assembling the blot -- 1.2.1 Cutting and preparing the membrane -- 1.2.2 Assembly and blotting -- 1.3 Dismantling the blot -- 1.4 Checking the efficiency of transfer -- 1.5 Fixing the DNA to the membrane -- 1.5.1 UV treatment -- 1.5.2 Drying -- 1.6 Storing membranes before hybridization -- 2. Capillary blotting on to multiple membranes at neutral pH |
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2.1 Uni-directional capillary blotting on to several membranes -- 2.2 Bi-directional capillary blotting -- 3. Capillary blotting at alkaline pH -- 4. Other methods of blotting -- 4.1 Electrophoretic transfer (electroblotting) -- 4.2 Vacuum blotting and positive-pressure blotting -- 4.3 Which method of blotting should you use? -- 5. Further reading -- 6. References -- Chapter 4 Electrophoresis of RNA and northern blotting -- 1. How does northern blotting differ from Southern blotting? -- 2. What information can a northern blot give? |
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3. Comparing the levels of an mRNA species in different cell types -- 3.1 Equal loading -- 3.2. Quantitation -- 3.2.1 Scintillation counting of excised bands -- 3.2.2 Scanning densitometry -- 3.2.3 Phosphorimagery -- 4. Gel electrophoresis of RNA samples -- 4.1 Gel systems -- 4.2 Formaldehyde gels -- 4.2.1 Preparing formaldehyde gels -- 4.2.2 Preparing RNA samples for formaldehyde gels -- 4.2.3 How much RNA should be loaded? -- 4.2.4 Running formaldehyde gels -- 4.3 Glyoxal gels -- 4.3.1 Preparing glyoxal gels -- 4.3.2 Preparing RNA samples for glyoxal gels -- 4.3.3 Running glyoxal gels |
| Notes |
Description based upon print version of record |
| Form |
Electronic book
|
| Author |
Bricknell, P. M
|
| ISBN |
9781000142778 |
|
1000142779 |
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