| Description |
1 online resource (248 p.) |
| Contents |
Cover -- Half Title -- Title -- Copyright -- Contents -- Preface -- List of Symbols -- Greek Letters -- Laboratory Report Format -- 1 Layout of a Fermenter with Controller -- 1.1 Introduction -- 1.2 Accessories of Fermenter -- 1.2.1 Aeration System -- 1.2.2 Mechanical Seal -- 1.2.3 Fermenter Vessel -- 1.2.4 Feed Ports -- 1.2.5 Baffles -- 1.2.6 Impeller -- 1.2.7 Sparger -- 1.2.8 Outlet -- 1.2.9 Safety Valve -- 1.3 Temperature Loop -- 1.3.1 Heater -- 1.3.2 Cold Finger -- 1.3.3 Solenoid Valve -- 1.4 Monitoring and Control Loop -- 1.4.1 Temperature Probe -- 1.4.2 pH Probe -- 1.4.3 O2 Sensor |
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1.4.4 Motor -- 1.5 Peristaltic Pump -- 1.6 Foam Control -- 2 Enzymatic Reaction Kinetics -- 2.1 Enzyme Activity -- 2.1.1 Alpha-Amylase Activity -- 2.2 Enzymatic Reaction Kinetics -- 2.2.1 Michaelis-Menten Kinetics -- 2.2.2 Limitation of the Michaelis-Menten Equation -- 2.2.3 Determination of Kinetic Constants -- 2.3 Calibration Curve of the Substrate -- 2.4 Preparation of Enzyme Solution -- 2.4.1 Procedure -- 2.5 Observation -- 2.6 Results -- 2.6.1 Sample Calculations -- 2.7 Discussion -- 3 Kinetics of the Immobilized Enzymatic Reaction -- 3.1 Preparation of Enzyme Solution |
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3.1.1 Definition of Enzyme Immobilization -- 3.2 Kinetic Theory -- 3.2.1 Effectiveness Factor and Damköhler Number -- 3.2.1.1 Determination of the factor affectsover all rate of the reaction -- 3.3 Preparation of Immobilized Enzyme -- 3.3.1 Preparation of Enzymatic Reaction Mixture -- 3.4 Observation -- 3.5 Calculated Data -- 3.6 Discussion -- 4 Cell Growth Kinetics in Controlled Fermenter -- 4.1 Introduction -- 4.2 Theory -- 4.3 Data Table -- 4.3.1 Calculated Data -- 4A Cell Growth Kinetics of Bacteria in a Batch Process -- 4A.1 Purpose -- 4A.2 Apparatus Required -- 4A.3 Medium Preparation |
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4A.3.1 Composition of the Medium -- 4A.3.2 Procedure for Medium Preparation -- 4A.3.3 Procedure for the Operation of the Fermenter -- 4A.4 Determination of Substrate Concentration -- 4A.4.1 Glucose Estimation by Dinitrosalicylic Acid (DNS) -- 4A.4.2 Determination of Cell Mass Concentration -- 4A.5 Observation -- 4A.6 Calculated Data -- 4A.6.1 Sample Calculation -- 4A.7 Discussion -- 4A.7.1 Possible Effect of Contamination of the Culture by an Organism with Value of Doubling Time of the Cell, td -- 4A.7.2 Contamination Problems -- 4A.7.3 Difference between Km and Ks -- 4A.8 Conclusion |
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4B Cell Growth Kinetics of Yeast in a Batch Process -- 4B.1 Purpose -- 4B.2 Background Statement -- 4B.3 Apparatus Required -- 4B.4 Medium Preparation -- 4B.5 Culture Preparation -- 4B.5.1 Procedure -- 4B.5.2 Cell Mass Concentration Estimation -- 4B.5.3 Glucose Estimation -- 4B.6 Observation -- 4B.7 Calculated Data -- 4B.7.1 Sample Calculation -- 4B.8 Discussion -- 4B.8.1 Contamination Problems -- 4B.8.2 Issue of Alcoholic Fermentation -- 4B.9 Conclusion -- 4C Cell Growth Kinetics of Microalgae in a Batch Process -- 4C.1 Purpose -- 4C.2 Background Statement -- 4C.2.1 Modes of Growth |
| Notes |
Description based upon print version of record |
| Form |
Electronic book
|
| Author |
Das, Debayan
|
| ISBN |
9781000225396 |
|
1000225399 |
|
