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Title Circular RNAs : methods and protocols / edited by Christoph Dieterich, Argyris Papantonis
Published New York, NY : Humana Press : Springer, [2018]
©2018

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Description 1 online resource (xiii, 220 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1724
Methods in molecular biology (Clifton, N.J.) ; v. 1724. 1064-3745
Contents Detection and reconstruction of circular RNAs from transcriptomic data / Yi Zheng and Fangqing Zhao -- Deep computational circular RNA analytics from RNA-seq data / Tobias Jakobi and Christoph Dieterich -- Genome-wide circRNA profiling from RNA-seq data / Daphne A. Cooper, Mariela Cortés-López, and Pedro Miura -- Analysis of circular RNAs using the web tool CircInteractome / Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, and Myriam Gorospe -- Characterization and validation of circular RNA and their host gene mRNA expression using PCR / Andreas W. Heumüller and Jes-Niels Boeckel -- Detecting circular RNAs by RNA fluorescence in situ hybridization / Anne Zirkel and Argyris Papantonis -- Single-molecule fluorescence in situ hybridization (FISH) of circular RNA CDR1as / Christine Kocks, Anastasiya Boltengagen, Monika Piwecka, Agnieszka Rybak-Wolf, and Nikolaus Rajewsky -- Highly efficient strategy for overexpressing circRNAs / Dawei Liu, Vanessa Conn, Gregory J. Goodall, and Simon J. Conn -- Constructing GFP-based reporter to study back splicing and translation of circular RNA / Yun Yang and Zefeng Wang -- Northern blot analysis of circular RNAs / Tim Schneider, Silke Schreiner, Christian Preusser, Albrecht Bindereif, and Oliver Rossbach -- Nonradioactive northern blot of circRNAs / Xiaolin Wang and Ge Shan -- Characterization of circular RNA concatemers / Thomas B. Hansen -- Characterization of circular RNAs (circRNA) associated with the translation machinery / Deniz Bartsch, Anne Zirkel, and Leo Kurian -- Synthesis and engineering of circular RNAs / Sonja Petkovic and Sabine Müller -- Preparation of circular RNA in vitro / Naoko Abe, Ayumi Kodama, and Hiroshi Abe -- Discovering circRNA-microRNA interactions from CLIP-seq data / Xiao-Qin Zhang and Jian-Hua Yang -- Identification of circRNAs for miRNA targets by argonaute2 RNA immunoprecipitation and luciferase screening assays / Yan Li, Bing Chen, and Shenglin Huang
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed January 11, 2018)
Subject RNA -- Laboratory manuals
RNA.
RNA
Medical genetics.
Medical -- Genetics.
RNA
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Dieterich, Christoph, editor
Papantonis, Argyris, editor
ISBN 9781493975624
1493975625