| Description |
1 online resource (2 volumes) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 363-364 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 363-364
|
| Contents |
v. 1. Preparation and crystallization of macromolecules -- v. 2. Structure determination |
|
Contents of Volume 1 -- A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag / Joseph E. Tropea [and others] -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project / Sophie Quevillon-Cheruel [and others] -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies / Dean R. Madden and Markus Safferling -- Protein Engineering / Sonia, Longhi, Francois Ferron, and Marie-Pierre Egloff -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems / Sylvie Doublié -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals / Gloria E.O. Borgstahl -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories / Terese Bergfors -- Improving Marginal Crystals / Charles W. Carter, Jr. and Madeleine Riès-Kautt -- Optimization Techniques for Automation and High Throughput / Naomi E. Chayen -- Three-Dimensional Crystallization of Membrane Proteins / James Féthière -- Crystallization of Protein-DNA Complexes / Thomas Hollis -- Preparation and Crystallization of RNA / Barbara L. Golden -- Crystallization of RNA-Protein Complexes / Eiji Obayashi [and others] -- Index |
|
Contents of Volume 2 -- Cryocrystallography of Macromolecules: Practice and Optimization / Elspeth Garman and Robin L. Owen -- Determination of Reaction Intermediate Structures in Heme Proteins / Kelvin Chu -- Annealing Macromolecular Crystals / B. Lief Hanson and Gerard J. Bunick -- First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction / David Jeruzalmi -- X-Ray Data Collection from Macromolecular Crystals / Elspeth Garman and Robert M. Sweet -- Characterizing a Crystal from an Initial Native Dataset / Michael R. Sawaya -- Molecular Replacement / Eric A. Toth -- Phase Determination using Halide Ions / Miroslawa Dauter and Zbigniew Dauter -- The Same But Different: Isomorphous Methods for Phasing and High-Throughput Ligand Screening / Mark A. Rould -- Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement with Anomalous Scattering Data Using Shake-and-Bake / G. David Smith, Christopher T. Lemke, and P. Lynne Howell -- Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments / Ralf W. Grosse-Kunstleve and Thomas R. Schneider -- Automated Structure Solution with autoSHARP / Clemens Vonrhein, Eric Blanc, Pietro Roversi, and Gérard Bricogne -- Introduction to Macromolecular Refinement / Dale E. Tronrud -- Quality Control and Validation / Gerard J. Kleywegt -- Crystallographic Software: A Sustainable Resource for the Community / Stephen J. Everse and Sylvie Doublié -- Index |
| Summary |
In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Macromolecules -- Structure
|
|
Crystallography.
|
|
Proteins -- Structure
|
|
Membrane proteins.
|
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X-ray crystallography -- Technique.
|
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Macromolecular Substances
|
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Crystallography
|
|
Membrane Proteins
|
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Proteins -- Structure.
|
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Membrane proteins.
|
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X-ray crystallography -- Technique.
|
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Proteins -- ultrastructure.
|
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Crystallography -- methods.
|
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Macromolecular Substances.
|
|
Nucleic Acids -- ultrastructure.
|
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Macromolecules -- Structure.
|
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Crystallography.
|
|
Crystallography
|
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Macromolecules -- Structure
|
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Membrane proteins
|
|
Proteins -- Structure
|
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X-ray crystallography -- Technique
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Doublié, Sylvie.
|
| ISBN |
1588292924 |
|
9781588292926 |
|
1588299023 |
|
9781588299024 |
|
9781597452090 |
|
1597452092 |
|
9781597452663 |
|
1597452661 |
|
