| Description |
1 online resource (xi, 251 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, 1940-6029 ; Volume 1192 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1192. 1064-3745
|
| Contents |
PART I. INTRODUCTION & HISTORICAL BACKGROUND -- 1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease / Ana Olivera and Juan Rivera -- PART II. OBTAINING THE CELLS: PURIFICATION, CULTURE, COUNTING, AND CELL LINES -- 2. Purification of Basophils from Peripheral Human Blood / Franco H. Falcone and Bernhard F. Gibbs -- 3. Mast Cell Purification Protocols / Jasmine Farrington, Elizabeth P. Seward, and Peter T. Peachell -- 4. Generation of a Human Allergic Mast Cell Phenotype from CD133 Stem Cells / Hans Jürgen Hoffmann -- 5. Generation of Mast Cells from Murine Stem Cell Progenitors / Emily J. Swindle -- 6. Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model / Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc -- 7. The Absolute Basophil Count / Elena Borzova and Clemens A. Dahinden -- 8. Mast Cell and Basophil Cell Lines: A Compendium / Egle Passante -- PART III. WHOLE CELLS: IDENTIFICATION IN TISSUES, SIGNALING PATHWAYS, GENE SILENCING, AND REPORTERS -- 9. Detection of Mast Cells and Basophils by Immunohistochemistry / Andrew F. Walls and Cornelia Amalinei -- 10. Measuring Histamine and Cytokine Release from Basophils and Mast Cells / Bettina M. Jensen, Sidsel Falkencrone, and Per S. Skov -- 11. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques / Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo -- 12. Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis / Monika Bambousková, Zuzana Hájková, Pavel Dráber, and Petr Dráber -- 13. Use of Humanized Rat Basophil Leukemia (RBL) Reporter Systems for Detection of Allergen-Specific IgE Sensitization in Human Serum / Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone -- 14. Gene Silencing Approaches in Mast Cells and Primary Human Basophils / Vadim V. Sumbayev and Bernhard F. Gibbs -- 15. Basophil Stimulation and Signaling Pathways / Edward F. Knol and Bernhard F. Gibbs -- 16. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells / José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, and Luís Escribano -- PART IV. WHOLE ORGANISM: DISEASE AND KNOCKOUT MODELS -- 17. Identification of Murine Basophils by Flow Cytometry and Histology / Christian Schwartz and David Voehringer -- 18. Mast Cell-Mediated Reactions In Vivo / Vladimir Andrey Giménez-Rivera, Martin Metz, and Frank Siebenhaar |
| Summary |
"In Basophils and Mast Cells: Methods and Protocols, experts in this challenging field explore techniques to research these cells from the most practical point of view. Given the tremendous influence of mast cells and blood-borne basophils over immune system function, this volume intends to aid the reader in the development of better tools for the isolation of these cells from primary tissues, peripheral blood, bone marrow, or cord blood. Also covered are protocols for the in vitro and in vivo study of their functions."--Publisher's description |
| Analysis |
biomedische wetenschappen |
|
biomedicine |
|
immunologie |
|
immunology |
|
Medicine (General) |
|
Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 25, 2014) |
| Subject |
Basophils -- Laboratory manuals
|
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Mast cells -- Laboratory manuals
|
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Immunology -- Laboratory manuals
|
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Basophils.
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Mast cells.
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Basophils
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Mast Cells
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Immunomodulation
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Basophils
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Mast cells
|
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Immunology
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Gibbs, Bernhard F., editor
|
|
Falcone, Franco H., editor
|
| LC no. |
2014944733 |
| ISBN |
9781493911738 |
|
1493911732 |
|
1493911724 |
|
9781493911721 |
|
