| Description |
1 online resource (x, 299 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1122 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1122. 1064-3745
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| Contents |
Advances in Research on Metalloproteins -- Purification of O2-Sensitive Metalloproteins -- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources -- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins -- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers -- Electrochemistry of Metalloproteins Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 -- FTIR Spectroscopy of Metalloproteins -- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy -- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds -- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) -- Mssbauer Spectroscopy -- X-Ray Absorption Spectroscopy of Metalloproteins -- X-Ray Crystallographic Studies of Metalloproteins -- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds -- Density Functional Theory Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups |
| Summary |
Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Metalloproteins.
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Proteins.
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Publication Formats
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Proteins
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Amino Acids, Peptides, and Proteins
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Publication Characteristics
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Chemicals and Drugs
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Metalloproteins
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Laboratory Manuals
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protein.
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Proteins
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Metalloproteins
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Fontecilla-Camps, Juan C., editor.
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Nicolet, Yvain, editor.
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| ISBN |
9781627037945 |
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1627037942 |
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1627037934 |
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9781627037938 |
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