Title Molecular dermatology : methods and protocols / edited by Cristina Has and Cassian Sitaru

Published New York : Humana Press, ©2013

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Description 1 online resource (xii, 460 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 961 Methods in molecular biology (Clifton, N.J.) ; v. 961. 1064-3745 Contents Molecular dermatology comes of age / Cristina Has and Cassian Sitaru -- Molecular diagnosis of autoimmune blistering diseases / Daisuke Tsuruta [and others] -- Molecular diagnosis of genodermatoses / Vesarat Wessagowit -- Three-dimensional visualization of the molecular architecture of cell-cell junctions in situ by cryo-electron tomography of vitreous sections / Ashraf Al-Amoudi and Achilleas S. Frangakis -- 3D visualization of epidermal Langerhans cells / Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai -- In vivo imaging of lymph node lymphangiogenesis by immuno-positron emission tomography / Viviane Mumprecht and Michael Detmar -- Optical imaging of HPV infection in a murine model / Rhonda C. Kines [and others] -- Laser scanning microscopy approach for semiquantitation of in vitro dermal particle penetration / Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider -- Analysis of cutaneous somatic mosaicism / Anna M.G. Pasmooij and Marcel F. Jonkman -- Global proteome analyses of SILAC-gabeled skin cells / Adrian Sprenger [and others] -- MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method / Janosch Hildebrand [and others] -- Cell death in the skin : how to study its quality and quantity? / Roman Makarov [and others] -- In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus / Ken Ishii and Masayuki Amagai -- Induction of granulocyte-dependent dermal-epidermal separation by autoantibodies ex vivo / Mircea Teodor Chiriac -- Analysis of collective invasion of carcinoma cells in a 3D organotypic model / Jean Albrengues, Gerrino Meneguzzi, and Cedric Gaggioli -- Isolation of melanoma tumor-initiating cells from surgical tissues / Alexander D. Boiko -- Detection, enumeration, and characterization of immune cells infiltrating melanoma tumors / Benjamin Toh [and others] -- Analysis of cell movement between skin and other anatomical sites in vivo using photoconvertible fluorescent protein "Kaede"-transgenic mice / Michio Tomura and Kenji Kabashima -- Reconstitution of skin fibrosis development using a tissue engineering approach / Véronique J. Moulin -- Bioengineered skin humanized model of psoriasis / Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Río -- Induction of contact hypersensitivity in the mouse model / Stefan F. Martin --Generation of functional multipotent keratinocytes from mouse induced and pluripotent stem cells / Ganna Bilousova and Dennis R. Roop -- RNAi-mediated gene function analysis in skin / Slobodan Beronja and Elaine Fuchs -- Mouse models of autoimmune blistering diseases induced by the passive transfer of antibodies / Wataru Nishie -- induction of experimental epidermolysis bullosa acquisita by immunization with murine collagen VII / Alina Sesarman and Cassian Sitaru -- Animal model for cutaneous leishmaniasis / Michael R. Fischer [and others] -- Model for generation of large numbers of primary inflammatory skin-derived neutrophils and macrophages / Dominik John, Michael R. Fischer, and Esther von Stebut -- Zebrafish as a model system to study heritable skin diseases / Qiaoli Li and Jouni Uitto -- Cell- and protein-based therapy approaches for epidermolysis bullosa / Alexander Nyström, Leena Bruckner-Tuderman, and Johannes S. Kern -- RNA trans-splicing for genodermatoses / Johann W. Bauer [and others] Summary The sustained skin research efforts over the past decades has led to the accumulation of a significant collection of information on skin structure and physiology as well as on the pathogenesis of cutaneous diseases. In Molecular Dermatology: Methods and Protocols, leading experts in the field provide a collection of state-of-the-art reliable protocols covering a wide spectrum of techniques and experimental models, specific molecular assays and disease models, as well as overviews of diagnostic and research areas relevant to molecular dermatology. As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Molecular Dermatology: Methods and Protocols emphasizes the vital importance of skin research and collects the methodologies necessary to aid scientists in moving forward in this valuable field Bibliography Includes bibliographical references and index Notes English Subject Skin -- Diseases. Dermatology, Experimental -- Laboratory manuals Biochemistry. Biology. Life sciences. Chemistry. Diseases. Medical personnel. Physical sciences. Genetics -- Technique. Diagnosis. Medicine Analytical, Diagnostic and Therapeutic Techniques and Equipment Biochemistry Publication Formats Investigative Techniques Skin and Connective Tissue Diseases Biology Biological Science Disciplines Publication Characteristics Chemistry Disease Health Occupations Natural Science Disciplines Disciplines and Occupations Genetics Genetic Techniques Molecular Biology Pathology Laboratory Manuals Skin Diseases Diagnosis biochemistry. biology. biological sciences. chemistry. physical sciences. diagnosis. Physical sciences Medical personnel Life sciences Genetics -- Technique Diseases Diagnosis Chemistry Biology Biochemistry Dermatology, Experimental Skin -- Diseases Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Has, Cristina. Sitaru, Cassian. ISBN 9781627032278 1627032274 1627032266 9781627032261

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