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Author Heil, John R

Title Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description online resource (streaming video file)(501 seconds)
Series Bioengineering
Summary A quick and efficient method to integrate foreign DNA of interest into pre-made acceptor strains, termed landing pad strains, is described. The method allows site-specific integration of a DNA cassette into the engineered landing pad locus of a given strain, through conjugation and expression of the ΦC31 integrase
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Bioengineering
Form Streaming video