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Author Herb, Marc

Title Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (406 secs.)
Series Immunology and Infection
Summary Macrophages, especially primary macrophages, are challenging to transfect as they specialize in detecting molecules of non-self origin. We describe a protocol that allows highly efficient transfection of primary macrophages with mRNA generated from DNA templates such as plasmids
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Immunology and Infection
Form Streaming audio