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Title Protocols for nucleic acid analysis by nonradioactive probes
Edition 2nd ed. / edited by Elena Hilario, John Mackay
Published Totowa, N.J. : Humana Press, ©2007

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Description 1 online resource (x, 321) : illustrations
Series Methods in molecular biology, 1064-3745 ; 353
Methods in molecular biology (Clifton, N.J.) ; v. 353
Contents Genomic DNA isolation from different biological materials / Duckchul Park -- Extraction of plant RNA / Elspeth MacRae -- Overview of hybridization and detection techniques / Elena Hilario -- Checkerboard DNA-DNA hybridization technology : using digoxigenin detection / Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons -- Nonradioactive northern and southern analyses from plant samples / Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye -- Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes / Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink -- Direct in-gel hybridization of DNA with digoxigenin-labeled probes / Saeed A. Khan and Mohamed S. Nawaz
In situ hybridization of termite microbes / Shigeharu Moriya [and others] -- RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer / Yukinori Eguchi -- Comparative quantitation of mRNA expression in the central nervous system using fluorescence in situ hybridization / Darren J. Day, Eli M. Mrkusich, and John H. Miller -- Visualization of gene expression by fluorescent multiplex reverse transcriptase-PCR amplification / María Rosa Ponce [and others] -- Fluorescence in situ hybridization for the identification of environmental microbes / Annelie Pernthaler and Jakob Pernthaler -- Introduction to kinetic ("real-time") PCR / John Mackay -- Validation of short interfering RNA knockdowns by quantitative real-time PCR / Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses
Real-time quantitative PCR as an alternative to southern blot or fluorescence in situ hybridization for detection of gene-copy number changes / Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele -- Design and work-up of a new molecular diagnostic assay based on real-time PCR / Harald H. Kessler -- Real-time PCR fluorescent chemistries / John Mackay and Olfert Landt -- Microarrays : an overview / Norman H. Lee and Alexander I. Saeed -- Oligonucleotide microarrays for the study of coastal microbial communities / Gaspar Taroncher-Oldenburg and Bess B. Ward
Summary Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Nucleic acid probes.
Nucleic acids -- Analysis.
DNA probes.
Nucleic Acid Amplification Techniques
DNA -- analysis
DNA Probes
Molecular Probe Techniques
SCIENCE -- Life Sciences -- Genetics & Genomics.
DNA -- Analysis.
DNA probes.
Molecular Probe Techniques.
Nucleic Acid Amplification Techniques.
Nucleic acid probes.
Nucleic acids -- Analysis.
DNA probes
Nucleic acid probes
Nucleic acids -- Analysis
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hilario, Elena, 1966-
Mackay, John, 1969-
LC no. 2006003449
ISBN 1588294307
9781588294302
1597452297
9781597452298
1280832703
9781280832703
9786610832705
6610832706
9781617375644
1617375640